http://rosebayblog.blogspot.com/2011/01/

H: Monday, January 31, 2011

Rhododendron austrinum is a Tetraploid

Rhododendron austrinum has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron austrinum was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Tetraploid Rhododendron austrinum,
Tetraploid Rhododendron austrinum Album Form,
Tetraploid Rhododendron austrinum 'Millie Mac'

Source: Arnold Arboretum Accession Number: 1403-86-A
Source: Connecticut College Arboretum Accession Number: 81-79-C
Source: Highstead Arboretum Accession Number: 93-79-K

John and Sally Perkins

H: Sunday, January 30, 2011

Rhododendron calendulaceum is a Tetraploid

Rhododendron calendulaceum has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron calendulaceum was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron calendulaceum var. croceum,
Tetraploid Rhododendron calendulaceum 'Cherokee',
Tetraploid Rhododendron calendulaceum 'Smoky Mountaineer'

Source: Longwood Garden Accession Number: 2001-0273-B
Source: Connecticut College Arboretum Accession Number: 80-7-D
Source: Connecticut College Arboretum Accession Number: 81-71-J
Source: Highstead Arboretum Accession Number: 93-80-G
Source: Arnold Arboretum Accession Number: 857-2-A
Source: Audra State Park, West Virginia
Source: Joe Bruso

John and Sally Perkins

H: Saturday, January 29, 2011

Reminder: P4M Workday: Planeview Nursery - Pot Cuttings @ Sat Feb 5 10am - 12pm (ARS Massachusetts Chapter Calendar)

P4M Workday: Planeview Nursery - Pot Cuttings

Stick Cuttings
When
Sat Feb 5 10am – 12pm Eastern Time
Where
770 Wapping Road, Portsmouth, RI (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

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Rhododendron alabamense Yellow Form is a Diploid

A yellow form of Rhododendron alabamense has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron alabamense was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron alabamense Yellow form

Source: John and Sally Perkins

John and Sally Perkins

H: Friday, January 28, 2011

Rhododendron flammeum 'Hazel Hamilton' is a Diploid

Rhododendron flammeum 'Hazel Hamilton' has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron flammeum was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron flammeun,
Diploid Rhododendron flammeum 'Brick Ruffles',
Diploid Rhododendron flammeum 'Harry's Speciosum',
Diploid Rhododendron flammeum 'Hazel Hamilton'

Source: John and Sally Perkins

John and Sally Perkins

H: Thursday, January 27, 2011

Rhododendron canescens is a Diploid

Rhododendron canescens has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron canescens was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron canescens

Source: Arnold Arboretum Accession Number: 166-90-B
Source: Highstead Arboretum Accession Number: 93-82-V
Source: Highstead Arboretum Accession Number: 93-82-W
Source: Longwood Gardens

John and Sally Perkins

H: Wednesday, January 26, 2011

Rhododendron arborescens is a Diploid

Rhododendron arborescens has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron arborescens was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron arborescens,
Diploid Rhododendron arborescens Pink Form,
Diploid Rhododendron arborescens Yellow Form Gable

Source: Arnold Arboretum Accession Number: 858-Mass
Source: Connecticut College Arboretum
Source: Audra State Park, West Virginia
Source: Joe Bruso

John and Sally Perkins

H: Tuesday, January 25, 2011

Rhododendron cumberlandense is a Diploid

Rhododendron cumberlandense has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron cumberlandense was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron cumberlandense

Source: Arnold Arboretum Accession Number: 419-97-D
Source: Connecticut College Arboretum Accession Number: 81-72-D
Source: Joe Bruso

John and Sally Perkins

H: Monday, January 24, 2011

Vermont Farm Rhododendron Walk

June 11, 2011 10 A.M. to Noon

Rhododendron Walk and Talk with Hal Bill, Collection Curator. A great opportunity to tour the Rhododendrons and Eastern U.S. Native Azalea collections in bloom. These “Jewels in the Crown” are one of best collections in New England. This event takes place at Friends of Hort Farm in Burlington, VT.

Rhododendron canadense is a Diploid

Rhododendron canadense has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron canadense was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch. The Ranney research involved several samples from diverse wild populations including samples from Salem, New Hampshire.

Read Ploidy Level of Rhododendron canadense

Summary of Ploidy Levels

Diploid Rhododendron canadense,
Diploid Rhododendron canadense var. album
Diploid Rhododendron canadense 'Osku's Showy'

Source: Arnold Arboretum Accession Number: 199-2006-C
Source: Bruce Clyburn
Source: John and Sally Perkins
Source: Kristian Theqvist

John and Sally Perkins

H: Sunday, January 23, 2011

Rhododendron molle is a Diploid

Rhododendron molle has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron molle was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron molle Orange,
Diploid Rhododendron molle Yellow,
Diploid Rhododendron molle ssp. japonicum,
Diploid Rhododendron molle ssp. molle

Source: Arnold Aboretum
Source: Joe Bruso
Source: George Hibben
Source: Longwood Gardens Accession Number: 1994-0068-B
Source: Kristian Theqvist

John and Sally Perkins

H: Saturday, January 22, 2011

Rhododendron vaseyi is a Diploid

Rhododendron vaseyi has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron vaseyi was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron vaseyi

Source: Arnold Arboretum Accession Number: 20953-Mass

John and Sally Perkins

Chapter Meeting Sunday at Elm Bank

Sunday, January 23, 1:30 PM we will meet at Elm Bank to hear biologist Richard Primack discuss his findings on the impact of recent climate fluctuations on local fauna and flora. 
See the most recent Newsletter for details.
 
Important Change:
Due to water damage in the Putnam Building we will meet in the East Wing of the Education Building.
 
Joe Bruso

H: Friday, January 21, 2011

Reminder: Massachusetts Chapter Meeting @ Sun Jan 23 1:30pm - 3:30pm (ARS Massachusetts Chapter Calendar)

Massachusetts Chapter Meeting

Meeting
When
Sun Jan 23 1:30pm – 3:30pm Eastern Time
Where
Elm Bank Wellsley, MA (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

You are receiving this email at the account john.a.perkins@gmail.com because you are subscribed for reminders on calendar ARS Massachusetts Chapter Calendar.

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Rhododendron eastmanii is a Diploid

Rhododendron eastmanii has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron eastmanii was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron eastmanii

Source: Joe Bruso

John and Sally Perkins

H: Thursday, January 20, 2011

Reminder: P4M Workday: Planeview Nursery - Pot Cuttings @ Sat Jan 22 10am - 12pm (ARS Massachusetts Chapter Calendar)

P4M Workday: Planeview Nursery - Pot Cuttings

Stick Cuttings
When
Sat Jan 22 10am – 12pm Eastern Time
Where
770 Wapping Road, Portsmouth, RI (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

You are receiving this email at the account john.a.perkins@gmail.com because you are subscribed for reminders on calendar ARS Massachusetts Chapter Calendar.

To stop receiving these notifications, please log in to https://www.google.com/calendar/ and change your notification settings for this calendar.

'Horizon Monarch' X 'L'Engin' is a Tetraploid

Five seedlings of Rhododendron 'Horizon Monarch' X 'L'Engin', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' (4X) X 'L'Engin' (4X), a tetraploid by a tetraploid produced a tetraploid indicating that normal meiosis most likely occurred for both tetraploid parents. In other words, the tetraploid seedlings of 'Horizon Monarch' X 'L'Engin' having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch' and 26 chromosomes from the tetraploid 'L'Engin'.

Summary of Ploidy Levels

Tetraploid 'Horizon Monarch',
Tetraploid 5 Seedlings of 'Horizon Monarch' X 'L'Engin',
Tetraploid 'L'Engin'

Source Marc Colombel

John and Sally Perkins

H: Wednesday, January 19, 2011

'Horizon Monarch' OP is a Tetraploid

One seedlings of Rhododendron 'Horizon Monarch' open pollinated, which was raised by Marc Colombel, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a tetraploid indicating that normal meiosis most likely occurred for the tetraploid seed parent. In other words, the tetraploid seedling of 'Horizon Monarch' open pollinated having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch'.

Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid.

Summary of Ploidy Levels

Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'

Source Marc Colombel

John and Sally Perkins

H: Tuesday, January 18, 2011

'Horizon Monarch' OP is a Diploid

Three seedlings of Rhododendron 'Horizon Monarch' open pollinated, which were raised by Marc Colombel, have been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the diploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a diploid indicating that supper reduced meiosis most likely occurred for the tetraploid seed parent. In other words, the diploid seedling of 'Horizon Monarch' open pollinated having 26 chromosomes received only 13 chromosomes from the tetraploid 'Horizon Monarch'. Tetraploids usually contribute 26 chromosomes to their offspring.

Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid. This provides direct evidence that a tetraploid seed parent can produce diploid offspring in a single generation.

Summary of Ploidy Levels

Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'

Source Marc Colombel

John and Sally Perkins

H: Monday, January 17, 2011

'Canobie Sunset' is a Triploid

Rhododendron 'Canobie Sunset', which is Sally and John Perkins introduction documented as 'Fedora' open pollinated, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Canobie Sunset' is an evergreen azalea.

Summary of Ploidy Levels

Triploid 'Canobie Sunset'

Source: John and Sally Perkins

John and Sally Perkins

H: Sunday, January 16, 2011

Reminder: Massachusetts Chapter Meeting @ Sun Jan 23 1:30pm - 3:30pm (ARS Massachusetts Chapter Calendar)

Massachusetts Chapter Meeting

Meeting
When
Sun Jan 23 1:30pm – 3:30pm Eastern Time
Where
Elm Bank Wellsley, MA (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

You are receiving this email at the account john.a.perkins@gmail.com because you are subscribed for reminders on calendar ARS Massachusetts Chapter Calendar.

To stop receiving these notifications, please log in to https://www.google.com/calendar/ and change your notification settings for this calendar.

Rhododendron periclymenoides is a Diploid

Rhododendron periclymenoides has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron periclymenoides tested was from a wild population located near Hamden, Connecticut.

Summary of Ploidy Levels

Diploid Rhododendron periclymenoides,
Diploid Rhododendron periclymenoides 'Appalachian Spring',
Diploid Rhododendron periclymenoides 'Doug Horegan'

Source: George Hibben
Source: Audra State Park, West Virginia
Source: Broken Arrow Nursery
Source: Connecticut College Arboretum Accession Number: 81-90-B
Source: Connecticut College Arboretum Accession Number: 81-90-E
Source: Longwood Gardens Accession Number: 2002-0208-B

John and Sally Perkins

H: Saturday, January 15, 2011

Reminder: P4M Workday: Planeview Nursery - Pot Cuttings @ Sat Jan 22 10am - 12pm (ARS Massachusetts Chapter Calendar)

P4M Workday: Planeview Nursery - Pot Cuttings

Stick Cuttings
When
Sat Jan 22 10am – 12pm Eastern Time
Where
770 Wapping Road, Portsmouth, RI (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

You are receiving this email at the account john.a.perkins@gmail.com because you are subscribed for reminders on calendar ARS Massachusetts Chapter Calendar.

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'Voluptuous' x 'Point Defiance' is a Triploid

A seedling of 'Voluptuous' x 'Point Defiance', which is a Werner Brack hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of this triploid seedling of 'Voluptuous' x 'Point Defiance', a diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, the triploid seedling having 39 chromosomes received 13 chromosomes from the diploid 'Voluptuous' and 26 chromosomes from the tetraploid 'Point Defiance'. Tetraploids usually contribute 26 chromosomes to their offspring.

Three seedlings were ploidy tested from the Brack cross of 'Voluptuous' x 'Point Defiance'. 'Orange Leopard' and 'Stony Brook' tested as diploid. This unnamed seedling tested as triploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

Summary of Ploidy Levels

Diploid 'Orange Leopard',
Diploid 'Stony Brook',
Diploid 'Voluptuous',
Triploid Seedling of 'Voluptuous' x 'Point Defiance',
Tetraploid 'Point Defiance'

Source: Werner Brack

John and Sally Perkins

H: Friday, January 14, 2011

'Orange Leopard' is a Diploid

Rhododendron 'Orange Leopard', which is a Werner Brack hybrid documented as 'Voluptuous' x 'Point Defiance', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of diploid 'Orange Leopard', a diploid crossed with a tetraploid produced a diploid indicating that supper reduced meiosis most likely occurred for the tetraploid parent. In other words, 'Orange Leopard' having 26 chromosomes received 13 chromosomes from the diploid 'Voluptuous' and 13 chromosomes from the tetraploid 'Point Defiance'. Tetraploids usually contribute 26 chromosomes to their offspring.

Three seedlings were ploidy tested from the Brack cross of 'Voluptuous' x 'Point Defiance'. 'Orange Leopard' and 'Stony Brook' tested as diploid. An unnamed seedling tested as triploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

Summary of Ploidy Levels

Diploid 'Orange Leopard',
Diploid 'Stony Brook',
Diploid 'Voluptuous',
Triploid Seedling of 'Voluptuous' x 'Point Defiance',
Tetraploid 'Point Defiance'

Source: Werner Brack

John and Sally Perkins

H: Thursday, January 13, 2011

'Stony Brook' is a Diploid

Rhododendron 'Stony Brook', which is a Werner Brack hybrid documented as 'Voluptuous' x 'Point Defiance', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of diploid 'Stony Brook', a diploid crossed with a tetraploid produced a diploid indicating that supper reduced meiosis most likely occurred for the tetraploid parent. In other words, 'Stony Brook' having 26 chromosomes received 13 chromosomes from the diploid 'Voluptuous' and 13 chromosomes from the tetraploid 'Point Defiance'. Tetraploids usually contribute 26 chromosomes to their offspring.

Three seedlings were ploidy tested from the Brack cross of 'Voluptuous' x 'Point Defiance'. 'Orange Leopard' and 'Stony Brook' tested as diploid. An unnamed seedling tested as triploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

Summary of Ploidy Levels

Diploid 'Orange Leopard',
Diploid 'Stony Brook',
Diploid 'Voluptuous',
Triploid Seedling of 'Voluptuous' x 'Point Defiance',
Tetraploid 'Point Defiance'

Source: Werner Brack

John and Sally Perkins

H: Wednesday, January 12, 2011

R. calendulaceum x occidentale is a Diploid

A seedling of Rhododendron calendulaceum x occidentale, which is a Oliver hybrid documented as R. calendulaceum 'Colossus' x R. occidentale, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

A seedling of Rhododendron calendulaceum x occidentale being a diploid may indicate the seed parent was R. cumberlandense instead of R. calendulaceum. This seedling was determined to be both seed and pollen fertile by John and Sally Perkins. Moreover, this deciduous azalea seedling pollen tested as a diploid by accepting pollen from confirmed diploids.

John and Sally Perkins suggest in the Rules of Engagement that tetraploid X diploid crosses in deciduous azaleas are seldom result in seedpods.

Rhododendron cumberlandense and Rhododendron occidentale are diploid species. Rhododendron calendulaceum is a tetraploid species.

Summary of Ploidy Levels

Diploid R. cumberlandense
Diploid R. occidentale
Diploid calendulaceum x occidentale
Tetraploid R. calendulaceum

Source: Dick Cavender

John and Sally Perkins

H: Tuesday, January 11, 2011

'Kehr's White Ruffles' is a Triploid

Rhododendron 'Kehr's White Ruffles', which is August Kehr hybrid documented as R. minus var. minus 'Epoch' x R. mucronulatum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Kehr's White Ruffles', a suspected tetraploid crossed with a diploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Kehr's White Ruffles' having 39 chromosomes received 26 chromosomes from the suspected tetraploid 'Epoch' and 13 chromosomes from the diploid R. mucronulatum.

Summary of Ploidy Levels

Triploid 'Angel Powder',
Triploid 'Kehr's White Ruffles'

Source: John and Sally Perkins

John and Sally Perkins

H: Monday, January 10, 2011

Reminder: Board Meeting @ Wed Jan 12 7:30pm - 9pm (ARS Massachusetts Chapter Calendar)

Board Meeting

When
Wed Jan 12 7:30pm – 9pm Eastern Time
Where
Hardings (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

You are receiving this email at the account john.a.perkins@gmail.com because you are subscribed for reminders on calendar ARS Massachusetts Chapter Calendar.

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'Angel Powder' is a Fertile Triploid

Rhododendron 'Angel Powder', which is Weldon Delp hybrid documented as R. minus var. minus 'Epoch' x R. mucronulatum var. mucronulatum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Werner Brack has germinated open pollinated seed collected from 'Angel Powder'. 'Angel Powder' is the first documented fertile triploid lepidote.

In the case of triploid 'Angel Powder', a tetraploid crossed with a diploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Angel Powder' having 39 chromosomes received 26 chromosomes from the tetraploid 'Epoch' and 13 chromosomes from the diploid R. mucronulatum.

Summary of Ploidy Levels

Triploid 'Angel Powder',
Triploid 'Kehr's White Ruffles',
Tetraploid R. minus var. minus 'Epoch'

Source: John and Sally Perkins

John and Sally Perkins

H: Sunday, January 09, 2011

'Le Fouesnantais' is a Tetraploid

Rhododendron 'Le Fouesnantais', which is a Marc Colombel hybrid documented as a 'Lem's Monarch' hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Tetraploid 'Le Fouesnantais',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster'

Source Marc Colombel

John and Sally Perkins

H: Saturday, January 08, 2011

'Markeeta’s Flame' is a Triploid

Rhododendron 'Markeeta’s Flame', which is Markeeta Nursery hybrid documented as 'Loderi Venus' x 'Anna', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Markeeta’s Flame', a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, 'Markeeta’s Flame' having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Rhododendron 'The Honorable Jean Marie de Montague' is known to throw unreduced gametes and Rhododendron 'Anna' has 'The Honorable Jean Marie de Montague' as a parent.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Loderi Venus',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Markeeta’s Flame',
Triploid 'Markeeta's Prize'

Source: Greer Gardens

John and Sally Perkins

H: Friday, January 07, 2011

January Newsletter

Here is the electronic January Newsletter, still not gussied up with pictures yet.  Hope to see most of you at the January Chapter Meeting on Sunday January 23 at Elm Bank in the Putnam Building at 1:30.   Richard Primack, Professor of Botany at BU, will be discussing his research on the local botanical/horticultural evidence of climate change and earlier bloom dates.  A big, important topic.

Susan Clark

'Markeeta's Prize' is a Triploid

Rhododendron 'Markeeta's Prize', which is Markeeta Nursery hybrid documented as 'Loderi Venus' x 'Anna', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Markeeta’s Prize', a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, 'Markeeta’s Prize' having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Rhododendron 'The Honorable Jean Marie de Montague' is known to throw unreduced gametes and Rhododendron 'Anna' has 'The Honorable Jean Marie de Montague' as a parent.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Loderi Venus',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Markeeta's Flame',
Triploid 'Markeeta's Prize'

Source: Greer Gardens

John and Sally Perkins

H: Thursday, January 06, 2011

'Lucky Strike' is a Triploid

Rhododendron 'Lucky Strike', which is Ted van Veen hybrid documented as R. griersonianum x 'Countess of Derby', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Lucky Strike', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Lucky Strike' having 39 chromosomes received 13 chromosomes from the suspected diploid R. griersonianum and 26 chromosomes from the tetraploid 'Countess of Derby'.

Summary of Ploidy Levels

Triploid 'Anna Rose Whitney',
Triploid 'Lucky Strike',
Triploid 'Van',
Tetraploid 'Countess of Derby'

Source: Van Veen Nursery

John and Sally Perkins

H: Wednesday, January 05, 2011

Reminder: Board Meeting @ Wed Jan 12 7:30pm - 9pm (ARS Massachusetts Chapter Calendar)

Board Meeting

When
Wed Jan 12 7:30pm – 9pm Eastern Time
Where
Hardings (map)
Calendar
ARS Massachusetts Chapter Calendar
Who
John Perkins - creator

Invitation from Google Calendar

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'Steredenn' is a Triploid

Rhododendron 'Steredenn', which is Marc Colombel hybrid documented as 'Kerneostic' x 'Lem's Monarch', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Steredenn', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Steredenn' having 39 chromosomes received 13 chromosomes from the suspected diploid 'Kerneostic' and 26 chromosomes from the tetraploid 'Lem's Monarch'.

Summary of Ploidy Levels

Triploid 'Steredenn',
Tetraploid 'Lem's Monarch'

Source Marc Colombel

John and Sally Perkins

H: Tuesday, January 04, 2011

'Lady of Spain' is a Triploid

Rhododendron 'Lady of Spain', which is Lofthouse hybrid documented as 'Mrs. Horace Fogg' x 'Point Defiance', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Lady of Spain', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Lady of Spain' having 39 chromosomes received 13 chromosomes from the suspected diploid 'Mrs. Horace Fogg' and 26 chromosomes from the tetraploid 'Point Defiance'.


Summary of Ploidy Levels

Triploid 'Lady of Spain',
Tetraploid 'Point Defiance'

Source Marc Colombel

John and Sally Perkins

H: Monday, January 03, 2011

'Countess of Athlone' is a Diploid

Rhododendron 'Countess of Athlone', which is a van Nes hybrid documented as 'Geoffroy Millais' x R. catawbiense 'Catawbiense Grandiflorum', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Countess of Athlone'

Source Marc Colombel

John and Sally Perkins

H: Sunday, January 02, 2011

'Red Olympia' is a Diploid

Rhododendron 'Red Olympia', which is a Halfdan Lem hybrid documented as 'Anna' x 'Fusilier Group', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Red Olympia',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague'

Source: Greer Gardens

John and Sally Perkins

H: Saturday, January 01, 2011

Rhododendron viscosum is a Diploid

Rhododendron viscosum has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron viscosum tested was from a wild population located in Salem, New Hampshire.

Rhododendron viscosum was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron viscosum,
Diploid Rhododendron viscosum forma rhodanthum,
Diploid Rhododendron viscosum var. serrulatum

Source: John and Sally Perkins

John and Sally Perkins
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