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Showing posts with label TetToTet. Show all posts
Showing posts with label TetToTet. Show all posts

H: Thursday, August 18, 2011

'Horizon Monarch' X 'Doreen Gale' is a Tetraploid

Seven seedlings of Rhododendron 'Horizon Monarch' X 'Doreen Gale', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' (4X) X 'Doreen Gale' (4X), a tetraploid by a tetraploid produced a tetraploid indicating that normal meiosis most likely occurred for both tetraploid parents. In other words, the tetraploid seedlings of 'Horizon Monarch' X 'Doreen Gale' having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch' and 26 chromosomes from the tetraploid 'Doreen Gale'.

Summary of Ploidy Levels

Tetraploid 'Doreen Gale',
Tetraploid 'Horizon Monarch',
Tetraploid 7 Seedlings of 'Horizon Monarch' X 'Doreen Gale'

Source Marc Colombel

John and Sally Perkins

H: Tuesday, August 16, 2011

'Rwain' X 'L'Engin' is a Tetraploid

Four seedlings of Rhododendron 'Rwain' X 'L'Engin', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Rwain' (3X) X 'L'Engin' (4X), a triploid by a tetraploid produced a tetraploid indicating that triploid seed parent 'Rwain' acted as a tetraploid. In other words, the tetraploid seedlings of 'Rwain' X 'L'Engin' having 52 chromosomes most likely received 26 chromosomes from the triploid 'Rwain' and 26 chromosomes from the tetraploid 'L'Engin'.

Summary of Ploidy Levels

Triploid 'Rwain',
Tetraploid 'L'Engin',
Tetraploid 4 Seedlings of 'Rwain' X 'L'Engin'

Source Marc Colombel

John and Sally Perkins

H: Saturday, August 13, 2011

'Horizon Monarch' X 'Rwain' is a Tetraploid

Three seedlings of Rhododendron 'Horizon Monarch' X 'Rwain', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' (4x) X 'Rwain' (3X), a tetraploid by a triploid produced a tetraploid indicating that triploid pollen parent 'Rwain' acted as a tetraploid. In other words, the tetraploid seedlings of 'Horizon Monarch' X 'Rwain' having 52 chromosomes most likely received 26 chromosomes from the tetraploid 'Horizon Monarch' and 26 chromosomes from the triploid 'Rwain'.

Summary of Ploidy Levels

Triploid 'Rwain',
Triploid 1 Seedling of 'Horizon Monarch' X 'Rwain'
Tetraploid 'Horizon Monarch',
Tetraploid 3 Seedlings of 'Horizon Monarch' X 'Rwain'

Source Marc Colombel

John and Sally Perkins

H: Thursday, July 28, 2011

'Horizon Monarch' X ('Lem's Monarch' X 'Very Berry')

One seedling of Rhododendron 'Horizon Monarch' X ('Lem's Monarch' X 'Very Berry'), which was raised by Marc Colombel, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' (4X) X ('Lem's Monarch' (4X) X 'Very Berry' (4X)), a tetraploid was produced having 3 triploid and 7 tetraploid ancestors.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Hotei',
Diploid 'Lem's Cameo',
Diploid 'Nancy Evans',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby',
Tetraploid 'Horizon Monarch',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Point Defiance'
Tetraploid 'Trude Webster',
Tetraploid 'Very Berry'
Tetraploid 1 Seedling of 'Horizon Monarch' X ('Lem's Monarch' X 'Very Berry')

Source Marc Colombel

John and Sally Perkins

H: Thursday, January 20, 2011

'Horizon Monarch' X 'L'Engin' is a Tetraploid

Five seedlings of Rhododendron 'Horizon Monarch' X 'L'Engin', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' (4X) X 'L'Engin' (4X), a tetraploid by a tetraploid produced a tetraploid indicating that normal meiosis most likely occurred for both tetraploid parents. In other words, the tetraploid seedlings of 'Horizon Monarch' X 'L'Engin' having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch' and 26 chromosomes from the tetraploid 'L'Engin'.

Summary of Ploidy Levels

Tetraploid 'Horizon Monarch',
Tetraploid 5 Seedlings of 'Horizon Monarch' X 'L'Engin',
Tetraploid 'L'Engin'

Source Marc Colombel

John and Sally Perkins

H: Wednesday, January 19, 2011

'Horizon Monarch' OP is a Tetraploid

One seedlings of Rhododendron 'Horizon Monarch' open pollinated, which was raised by Marc Colombel, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a tetraploid indicating that normal meiosis most likely occurred for the tetraploid seed parent. In other words, the tetraploid seedling of 'Horizon Monarch' open pollinated having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch'.

Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.

The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid.

Summary of Ploidy Levels

Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'

Source Marc Colombel

John and Sally Perkins

H: Sunday, January 09, 2011

'Le Fouesnantais' is a Tetraploid

Rhododendron 'Le Fouesnantais', which is a Marc Colombel hybrid documented as a 'Lem's Monarch' hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Tetraploid 'Le Fouesnantais',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster'

Source Marc Colombel

John and Sally Perkins

H: Monday, December 13, 2010

'Legend' is a Tetraploid

Rhododendron 'Legend', which is a Jim Barlup hybrid documented as 'Point Defiance' x 'Lem's Monarch', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Legend' has as ancestors 'Anna', 'Norman Gill', 'The Honourable Jean Marie de Montague', 'Marinus Koster', 'Point Defiance', and 'Lem's Monarch'.

In the case of the tetraploid 'Legend', a tetraploid crossed with a tetraploid produced a tetraploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Legend' having 52 chromosomes received 26 chromosomes from the tetraploid 'Point Defiance' and 26 chromosomes from the tetraploid 'Lem's Monarch'.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Tetraploid 'Legend',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Point Defiance'

Source: Whitney Nursery

John and Sally Perkins

H: Sunday, December 12, 2010

'Lem's Monarch' is a Tetraploid

Rhododendron 'Lem's Monarch', which is a Halfdan Lem hybrid documented as 'Anna' x 'Marinus Koster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Lem's Monarch' has as a descendants 'Rwain'.

In the case of the tetraploid 'Lem's Monarch', a diploid crossed with a tetraploid produced a tetraploid indicating that unreduced meiosis may have occurred for the diploid parent. In other words, 'Lem's Monarch' having 52 chromosomes received something similar to 26 chromosomes from the diploid 'Anna' and 26 chromosomes from the tetraploid 'Marinus Koster'.

Rhododendron 'Lem's Monarch' was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'El Camino',
Triploid 'Rwain',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Pink Walloper',
Tetraploid 'Point Defiance',
Tetraploid 'Walloper No. 10'

Source Marc Colombel

John and Sally Perkins

H: Thursday, December 09, 2010

'Doreen Gale' is a Tetraploid

Rhododendron 'Doreen Gale', which is a Sanders hybrid documented as 'Point Defiance' x 'Trude Webster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Doreen Gale' has as ancestors 'Anna', 'Norman Gill', 'The Honourable Jean Marie de Montague', 'Broughtonii', 'Cynthia', 'Pink Pearl', 'Marinus Koster', 'Point Defiance', 'Countess of Derby', and 'Trude Webster'.

In the case of the tetraploid 'Doreen Gale', a tetraploid crossed with a tetraploid produced a tetraploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Doreen Gale' having 52 chromosomes received 26 chromosomes from the tetraploid 'Point Defiance' and 26 chromosomes from the tetraploid 'Trude Webster'.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby',
Tetraploid 'Doreen Gale',
Tetraploid 'Gorgeous George',
Tetraploid 'Marinus Koster',
Tetraploid 'Point Defiance',
Tetraploid 'Trude Webster'

Source Marc Colombel

John and Sally Perkins

H: Tuesday, December 07, 2010

'Horizon Monarch' is a Tetraploid

Rhododendron 'Horizon Monarch', which is a Ned Brockenbrough hybrid documented as 'Nancy Evans' x 'Point Defiance', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Horizon Monarch' has as ancestors 'Anna', 'Hotei', 'Lem's Cameo', 'Nancy Evans', 'Norman Gill', 'The Honourable Jean Marie de Montague', 'Horizon Monarch', 'Marinus Koster', and 'Point Defiance'.

In the case of the tetraploid 'Horizon Monarch', a diploid crossed with a tetraploid produced a tetraploid indicating that unreduced meiosis may have occurred for the diploid parent. In other words, 'Horizon Monarch' having 52 chromosomes received something similar to 26 chromosomes from the diploid 'Nancy Evans' and 26 chromosomes from the tetraploid 'Point Defiance'.

Rhododendron 'Horizon Monarch' was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Hotei',
Diploid 'Lem's Cameo',
Diploid 'Nancy Evans',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'Steredenn',
Tetraploid 'Horizon Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Point Defiance'

Source Marc Colombel

John and Sally Perkins

H: Sunday, December 05, 2010

'Walloper No. 10' is a Tetraploid

Rhododendron 'Walloper No. 10', which is a Halfdan Lem hybrid documented as 'Anna' x 'Marinus Koster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the tetraploid 'Walloper No. 10', a diploid crossed with a tetraploid produced a tetraploid indicating that unreduced meiosis may have occurred for the diploid parent. In other words, 'Pink Walloper' having 52 chromosomes received something similar to 26 chromosomes from the diploid 'Anna' and 26 chromosomes from the tetraploid 'Marinus Koster'.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'El Camino',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Pink Walloper',
Tetraploid 'Point Defiance',
Tetraploid 'Walloper No. 10'

Source: Van Veen Nursery

John and Sally Perkins

H: Saturday, December 04, 2010

'Pink Walloper' is a Tetraploid

Rhododendron 'Pink Walloper', which is a Halfdan Lem hybrid documented as 'Anna' x 'Marinus Koster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Pink Walloper' has as a descendant 'Super Dog'.

In the case of the tetraploid 'Pink Walloper', a diploid crossed with a tetraploid produced a tetraploid indicating that unreduced meiosis may have occurred for the diploid parent. In other words, 'Pink Walloper' having 52 chromosomes received something similar to 26 chromosomes from the diploid 'Anna' and 26 chromosomes from the tetraploid 'Marinus Koster'.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'El Camino',
Triploid 'Super Dog',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Pink Walloper',
Tetraploid 'Point Defiance',
Tetraploid 'Walloper No. 10'

John and Sally Perkins

H: Friday, December 03, 2010

'Gorgeous George' is a Tetraploid

Rhododendron 'Gorgeous George', which is a Sanders hybrid documented as 'Point Defiance' x 'Trude Webster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Gorgeous George' has as ancestors 'Anna', 'Norman Gill', 'The Honourable Jean Marie de Montague', 'Broughtonii', 'Cynthia', 'Pink Pearl', 'Marinus Koster', 'Point Defiance', 'Countess of Derby', and 'Trude Webster'.

In the case of the tetraploid 'Gorgeous George', a tetraploid crossed with a tetraploid produced a tetraploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Gorgeous George' having 52 chromosomes received 26 chromosomes from the tetraploid 'Point Defiance' and 26 chromosomes from the tetraploid 'Trude Webster'.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby',
Tetraploid 'Doreen Gale',
Tetraploid 'Gorgeous George',
Tetraploid 'Marinus Koster',
Tetraploid 'Point Defiance',
Tetraploid 'Trude Webster'

Source: Whitney Nursery

John and Sally Perkins

H: Thursday, December 02, 2010

'Point Defiance' is a Tetraploid

Rhododendron 'Point Defiance', which is a Halfdan Lem hybrid documented as 'Anna' x 'Marinus Koster', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Point Defiance' has as descendants 'Doreen Gale', 'Gentle Giant', 'Horizon Monarch', 'Julia Caroline', 'Lady of Spain', 'Orange Leopard', and 'Stony Brook'.

In the case of the tetraploid 'Point Defiance', a diploid crossed with a tetraploid produced a tetraploid indicating that unreduced meiosis may have occurred for the diploid parent. In other words, 'Point Defiance' having 52 chromosomes received something similar to 26 chromosomes from the diploid 'Anna' and 26 chromosomes from the tetraploid 'Marinus Koster'.

Rhododendron 'Point Defiance' was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Horizon Lakeside',
Diploid 'Nancy Evans',
Diploid 'Norman Gill',
Diploid 'Orange Leopard',
Diploid 'Stony Brook',
Diploid 'The Honourable Jean Marie de Montague',
Triploid 'El Camino',
Triploid 'Julia Caroline',
Triploid 'Lady of Spain',
Triploid 'Platinum Pearl',
Tetraploid 'Doreen Gale',
Tetraploid 'Gentle Giant',
Tetraploid 'Horizon Monarch',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster',
Tetraploid 'Pink Walloper',
Tetraploid 'Point Defiance',
Tetrsploid 'Trude Webster',
Tetraploid 'Walloper No. 10'

Source: Greer Gardens

John and Sally Perkins

H: Friday, November 05, 2010

'Very Berry' is a Tetraploid

Rhododendron 'Very Berry', which is a Harold Greer hybrid documented as 'Trude Webster' x 'The Honorable Jean Marie de Montague', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Very Berry' has 'Broughtonii', 'Pink Pearl', 'Cynthia', 'Countess of Derby', 'Trude Webster', and 'The Honorable Jean Marie de Montague' as ancestors. 'Verry Berry' appears to be another case where 'The Honorable Jean Marie de Montague' threw unreduced gametes.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby',
Tetraploid 'Trude Webster',
Tetraploid 'Very Berry'

Source: Greer Gardens

John and Sally Perkins

H: Saturday, April 10, 2010

Chickasaw X Golden Comet



Here are Bruce Clyburn's seedlings of 'Chickasaw' X luteum 'Golden Comet' a cross by Sally and John Perkins.

'Chickasaw' is a "pollen sterile" tetraploid found by Clarence Towe in the wild and R. luteum 'Golden Comet' is a tetraploid selected by the Rhododendron Species Foundation.

'Chickasaw' was suspected of being a triploid but flow cytometry indicates 'Chickasaw' is a tetraploid.
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