Five seedlings of Rhododendron 'Horizon Monarch' X 'L'Engin', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the tetraploid seedling of 'Horizon Monarch' (4X) X 'L'Engin' (4X), a tetraploid by a tetraploid produced a tetraploid indicating that normal meiosis most likely occurred for both tetraploid parents. In other words, the tetraploid seedlings of 'Horizon Monarch' X 'L'Engin' having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch' and 26 chromosomes from the tetraploid 'L'Engin'.
Summary of Ploidy Levels
Tetraploid 'Horizon Monarch',
Tetraploid 5 Seedlings of 'Horizon Monarch' X 'L'Engin',
Tetraploid 'L'Engin'
Source Marc Colombel
John and Sally Perkins
Showing posts with label H_Colombel. Show all posts
Showing posts with label H_Colombel. Show all posts
H: Thursday, January 20, 2011
H: Wednesday, January 19, 2011
'Horizon Monarch' OP is a Tetraploid
One seedlings of Rhododendron 'Horizon Monarch' open pollinated, which was raised by Marc Colombel, has been determined to be a tetraploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the tetraploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a tetraploid indicating that normal meiosis most likely occurred for the tetraploid seed parent. In other words, the tetraploid seedling of 'Horizon Monarch' open pollinated having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch'.
Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.
The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid.
Summary of Ploidy Levels
Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'
Source Marc Colombel
John and Sally Perkins
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the tetraploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a tetraploid indicating that normal meiosis most likely occurred for the tetraploid seed parent. In other words, the tetraploid seedling of 'Horizon Monarch' open pollinated having 52 chromosomes received 26 chromosomes from the tetraploid 'Horizon Monarch'.
Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.
The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid.
Summary of Ploidy Levels
Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'
Source Marc Colombel
John and Sally Perkins
H: Tuesday, January 18, 2011
'Horizon Monarch' OP is a Diploid
Three seedlings of Rhododendron 'Horizon Monarch' open pollinated, which were raised by Marc Colombel, have been determined to be a diploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the diploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a diploid indicating that supper reduced meiosis most likely occurred for the tetraploid seed parent. In other words, the diploid seedling of 'Horizon Monarch' open pollinated having 26 chromosomes received only 13 chromosomes from the tetraploid 'Horizon Monarch'. Tetraploids usually contribute 26 chromosomes to their offspring.
Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.
The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid. This provides direct evidence that a tetraploid seed parent can produce diploid offspring in a single generation.
Summary of Ploidy Levels
Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'
Source Marc Colombel
John and Sally Perkins
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the diploid seedling of 'Horizon Monarch' open pollinated, a tetraploid seed parent produced a diploid indicating that supper reduced meiosis most likely occurred for the tetraploid seed parent. In other words, the diploid seedling of 'Horizon Monarch' open pollinated having 26 chromosomes received only 13 chromosomes from the tetraploid 'Horizon Monarch'. Tetraploids usually contribute 26 chromosomes to their offspring.
Four seedlings were ploidy tested from seed collected by Marc Colombel from 'Horizon Monarch'. Three tested as diploid and one seedling tested as tetraploid. This indicates that the offspring of a given cross can have different ploidy levels from one offspring to another.
The 'Horizon Monarch' used to collect open pollinated seed by Marc Colombel ploidy tested as a tetraploid. This provides direct evidence that a tetraploid seed parent can produce diploid offspring in a single generation.
Summary of Ploidy Levels
Diploid 3 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 1 Seedlings of 'Horizon Monarch' open pollinated,
Tetraploid 'Horizon Monarch'
Source Marc Colombel
John and Sally Perkins
H: Sunday, January 09, 2011
'Le Fouesnantais' is a Tetraploid
Rhododendron 'Le Fouesnantais', which is a Marc Colombel hybrid documented as a 'Lem's Monarch' hybrid, has been determined to be a tetraploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
Summary of Ploidy Levels
Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Tetraploid 'Le Fouesnantais',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster'
Source Marc Colombel
John and Sally Perkins
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
Summary of Ploidy Levels
Diploid 'Anna',
Diploid 'Norman Gill',
Diploid 'The Honourable Jean Marie de Montague',
Tetraploid 'Le Fouesnantais',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marinus Koster'
Source Marc Colombel
John and Sally Perkins
H: Wednesday, January 05, 2011
'Steredenn' is a Triploid
Rhododendron 'Steredenn', which is Marc Colombel hybrid documented as 'Kerneostic' x 'Lem's Monarch', has been determined to be a triploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of triploid 'Steredenn', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Steredenn' having 39 chromosomes received 13 chromosomes from the suspected diploid 'Kerneostic' and 26 chromosomes from the tetraploid 'Lem's Monarch'.
Summary of Ploidy Levels
Triploid 'Steredenn',
Tetraploid 'Lem's Monarch'
Source Marc Colombel
John and Sally Perkins
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of triploid 'Steredenn', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Steredenn' having 39 chromosomes received 13 chromosomes from the suspected diploid 'Kerneostic' and 26 chromosomes from the tetraploid 'Lem's Monarch'.
Summary of Ploidy Levels
Triploid 'Steredenn',
Tetraploid 'Lem's Monarch'
Source Marc Colombel
John and Sally Perkins
H: Sunday, October 03, 2010
'Rwain' is a Fertile Triploid
Rhododendron 'Rwain', which is a Marc Colombel hybrid documented as 'Kerneostic' X 'Lem's Monarch', has been determined to be a triploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
The University of Coimbra research team also determined the Colombel cross of 'Horizon Monarch' by 'Rwain' produced a triploid and 3 tetraploids, 'Hotei' x 'Rwain' produced a triploid, the Colombel cross of 'Rwain' X 'L'Engin' produced 4 tetraploids, and the Colombel cross of 'Naselle' X 'Rwain' produced a diploid.
Thus, 'Rwain' is a seed and pollen fertile triplod capable of producing offspring that are diploids, triploids, and tetraploids. Although 'Rwain' is fertile, crosses involving 'Rwain' normally produce very few viable seeds.
These findings suggest that for the genus Rhododendrons triploids may provide a path for the exchange of generic material between diploids and tetraploids. To date the evidence suggesting a flow from diploid to tetraploid is much stronger than the evidence for a path from tetraploid to diploid.
Uneven bell curve splitting of fertile triploids allowing triploids to act as a donor of 1x or 2x gametes is demonstrated to occur in the research done in the thirties by Dr. Cyril Dean Darlington and in the nineties by Dr. Justin Ramsey.
These findings are consistent with the observations that Jim Barlup and Marc Colombel made when working with suspected polyploid elepidotes and the rules of engagement of John and Sally Perkins when working with deciduous azaleas.
'Lem's Monarch' and 'Horizon Monarch' have been determined to be tetraploids using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.
"Chromosomes and Plant Breeding", Macmillan (1932).
"Neopolyploidy in Flowering Plants" (2002).
"Let’s talk hybridizing: Hybridizing with elepidote polyploid rhododendrons." (2002)
"Rules of Engagement" (2009)
"Ploidy Levels and Relative Genome Sizes of Diverse Species, Hybrids, and Cultivars of Rhododendron" (2006)
Summary of Ploidy levels
Diploid 'Anna',
Diplid 'Countess of Athlone',
Diploid 'Hotei',
Diploid 'Naselle',
Diploid 'Norman Gill' ,
Diploid 'The Honourable Jean Marie de Montague',
Diploid 1 seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 seedling of 'Horizon Monarch' x 'Rwain',
Triploid 1 seedling of 'Hotei' x 'Rwain',
Tetraploid 'Horizon Monarch',
Tetraploid 'L'Engin',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marnius Koster',
Tetraploid 3 seedlings of 'Horizon Monarch' x 'Rwain',
Tetraploid 4 seedlings of 'Rwain' X 'L'Engin'
Source Marc Colombel
Note: R W A I N is short for Rhododendron Without An Important Name
John and Sally Perkins
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
The University of Coimbra research team also determined the Colombel cross of 'Horizon Monarch' by 'Rwain' produced a triploid and 3 tetraploids, 'Hotei' x 'Rwain' produced a triploid, the Colombel cross of 'Rwain' X 'L'Engin' produced 4 tetraploids, and the Colombel cross of 'Naselle' X 'Rwain' produced a diploid.
Thus, 'Rwain' is a seed and pollen fertile triplod capable of producing offspring that are diploids, triploids, and tetraploids. Although 'Rwain' is fertile, crosses involving 'Rwain' normally produce very few viable seeds.
These findings suggest that for the genus Rhododendrons triploids may provide a path for the exchange of generic material between diploids and tetraploids. To date the evidence suggesting a flow from diploid to tetraploid is much stronger than the evidence for a path from tetraploid to diploid.
Uneven bell curve splitting of fertile triploids allowing triploids to act as a donor of 1x or 2x gametes is demonstrated to occur in the research done in the thirties by Dr. Cyril Dean Darlington and in the nineties by Dr. Justin Ramsey.
These findings are consistent with the observations that Jim Barlup and Marc Colombel made when working with suspected polyploid elepidotes and the rules of engagement of John and Sally Perkins when working with deciduous azaleas.
'Lem's Monarch' and 'Horizon Monarch' have been determined to be tetraploids using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.
"Chromosomes and Plant Breeding", Macmillan (1932).
"Neopolyploidy in Flowering Plants" (2002).
"Let’s talk hybridizing: Hybridizing with elepidote polyploid rhododendrons." (2002)
"Rules of Engagement" (2009)
"Ploidy Levels and Relative Genome Sizes of Diverse Species, Hybrids, and Cultivars of Rhododendron" (2006)
Summary of Ploidy levels
Diploid 'Anna',
Diplid 'Countess of Athlone',
Diploid 'Hotei',
Diploid 'Naselle',
Diploid 'Norman Gill' ,
Diploid 'The Honourable Jean Marie de Montague',
Diploid 1 seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 seedling of 'Horizon Monarch' x 'Rwain',
Triploid 1 seedling of 'Hotei' x 'Rwain',
Tetraploid 'Horizon Monarch',
Tetraploid 'L'Engin',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marnius Koster',
Tetraploid 3 seedlings of 'Horizon Monarch' x 'Rwain',
Tetraploid 4 seedlings of 'Rwain' X 'L'Engin'
Source Marc Colombel
Note: R W A I N is short for Rhododendron Without An Important Name
John and Sally Perkins
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