Four seedlings of Rhododendron 'Rwain' X 'L'Engin', which were raised by Marc Colombel, have been determined to be a tetraploid using flow cytometry by
Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.
In the case of the tetraploid seedling of 'Rwain' (3X) X 'L'Engin' (4X), a triploid by a tetraploid produced a tetraploid indicating that triploid seed parent 'Rwain' acted as a tetraploid. In other words, the tetraploid seedlings of 'Rwain' X 'L'Engin' having 52 chromosomes most likely received 26 chromosomes from the triploid 'Rwain' and 26 chromosomes from the tetraploid 'L'Engin'.
Summary of Ploidy Levels
Triploid 'Rwain',
Tetraploid 'L'Engin',
Tetraploid 4 Seedlings of 'Rwain' X 'L'Engin'
Source Marc Colombel
John and Sally Perkins
