H: Monday, August 15, 2011

'Naselle' X 'Rwain' is a Diploid

One seedling of Rhododendron 'Naselle' X 'Rwain', which was raised by Marc Colombel, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the diploid seedling of 'Naselle' (2X) X 'Rwain'(3X), a diploid by a triploid produced a diploid indicating that triploid pollen parent 'Rwain' acted as a diploid. In other words, the diploid seedlings of 'Naselle' X 'Rwain' having 26 chromosomes most likely received 13 chromosomes from the diploid 'Naselle' and 13 chromosomes from the triploid 'Rwain'.

Summary of Ploidy Levels

Diploid 'Hotei',
Diploid 'Naselle',
Diploid 1 Seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 Seedling of 'Hotei' x 'Rwain'

Source Marc Colombel

John and Sally Perkins
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