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Showing posts with label TripToDip. Show all posts
Showing posts with label TripToDip. Show all posts

H: Monday, August 15, 2011

'Naselle' X 'Rwain' is a Diploid

One seedling of Rhododendron 'Naselle' X 'Rwain', which was raised by Marc Colombel, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the diploid seedling of 'Naselle' (2X) X 'Rwain'(3X), a diploid by a triploid produced a diploid indicating that triploid pollen parent 'Rwain' acted as a diploid. In other words, the diploid seedlings of 'Naselle' X 'Rwain' having 26 chromosomes most likely received 13 chromosomes from the diploid 'Naselle' and 13 chromosomes from the triploid 'Rwain'.

Summary of Ploidy Levels

Diploid 'Hotei',
Diploid 'Naselle',
Diploid 1 Seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 Seedling of 'Hotei' x 'Rwain'

Source Marc Colombel

John and Sally Perkins

H: Thursday, November 25, 2010

'Summer Peach' is a Diploid

Rhododendron 'Summer Peach', which is a Jim Barlup hybrid documented as 'Whitney's Late Peach' X 'Phyllis Korn', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Summer Peach' has as ancestors 'Broughtonii', 'Pink Pearl', 'Gomer Waterer', and 'Phyllis Korn' which are all triploids. 'Summer Peach' has as descendants 'Peach Recital' and 'Summer Wind' which are both diploids.

Summary of Ploidy Levels

Diploid 'Diane',
Diploid 'Madame Carvalho',
Diploid 'Mrs. Lindsay Smith',
Diploid 'Peach Recital',
Diploid 'Summer Peach',
Diploid 'Summer Wind',
Triploid 'Broughtonii',
Triploid 'Gomer Waterer',
Triploid 'Phyllis Korn',
Triploid 'Pink Pearl'

Source: Whitney Nursery

John and Sally Perkins

H: Wednesday, November 24, 2010

'J.G. Millais' is a Diploid

Rhododendron 'J.G. Millais', which is a 1915 Waterer hybrid documented as 'Ascot Brilliant' x 'Pink Pearl', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'J.G. Millais' has as ancestors 'Broughtonii' and 'Pink Pearl' which are both triploids.

Summary of Ploidy Levels

Diploid 'J.G. Millais',
Triploid 'Broughtonii',
Triploid 'Pink Pearl'

Source: Van Veen Nursery

John and Sally Perkins

H: Tuesday, November 23, 2010

'Graf Zeppelin' is a Diploid

Rhododendron 'Graf Zeppelin', which is a C. B. Van Nes hybrid documented as 'Pink Pearl' x 'Mrs. Charles S. Sargent', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Graf Zeppelin' has as ancestors 'Broughtonii' and 'Pink Pearl' which are both triploids.

Summary of Ploidy Levels

Diploid 'Graf Zeppelin',
Triploid 'Broughtonii',
Triploid 'Pink Pearl'

Source: Van Veen Nursery
Source: Whitney Nursery

John and Sally Perkins

H: Tuesday, October 05, 2010

'Hurricane' is a Diploid

Rhododendron 'Hurricane', which is a William Whitney hybrid documented as 'Mrs Furnivall' x 'Anna Rose Whitney', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Hurricane' has as ancestors 'Broughtonii', 'Cynthia', 'Pink Pearl', 'Countess of Derby', 'Mrs Furnivall', and 'Anna Rose Whitney'.

In the case of 'Hurricane', a diploid 'Mrs. Furnivall' crossed with a triploid 'Anna Rose Whitney' produced a diploid indicating 'Anna Rose Whitney' most likely contributed only a 1x gamete (13 chromosomes) to 'Hurricane'.

Summary of Ploidy Levels

Diploid 'Hurricane',
Diploid 'Mrs. Furnivall',
Triploid 'Anna Rose Whitney',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby'

Source: Whitney Nursery

John and Sally Perkins
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