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Showing posts with label 2x2. Show all posts
Showing posts with label 2x2. Show all posts

H: Tuesday, November 01, 2011

'Lilac Lights' is a Diploid

Rhododendron 'Lilac Lights', which is in the Northern Lights group from the University of Minnesota documented as Rhododendron canadense X Rhododendron molle hybrid, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron canadense,
Diploid Rhododendron molle,
Diploid Rhododendron prinophyllum,
Diploid 'Frank Abbott',
Diploid 'Jane Abbott',
Diploid 'Lilac Lights',
Diploid 'Orchid Lights',
Diploid 'Rosy Lights',
Triploid 'Spicy Lights',
Triploid 'White Lights',
Tetraploid Rhododendron atlanticum,
Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron luteum,
Tetraploid 'Apricot Surprise',
Tetraploid 'Candy Lights',
Tetraploid 'Golden Lights',
Tetraploid 'Lemon Lights',
Tetraploid 'Mandarin Lights',
Tetraploid 'Northern Hi-Lights',
Tetraploid 'Tri-Lights'

Source: Kristian Theqvist

John and Sally Perkins

H: Friday, October 28, 2011

'Rosy Lights' is a Diploid

Rhododendron 'Rosy Lights', which is in the Northern Lights group from the University of Minnesota documented as Rhododendron prinophyllum X Rhododendron molle, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron canadense,
Diploid Rhododendron molle,
Diploid Rhododendron prinophyllum,
Diploid 'Frank Abbott',
Diploid 'Jane Abbott',
Diploid 'Lilac Lights',
Diploid 'Orchid Lights',
Diploid 'Rosy Lights',
Triploid 'Spicy Lights',
Triploid 'White Lights',
Tetraploid Rhododendron atlanticum,
Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron luteum,
Tetraploid 'Apricot Surprise',
Tetraploid 'Candy Lights',
Tetraploid 'Golden Lights',
Tetraploid 'Lemon Lights',
Tetraploid 'Mandarin Lights',
Tetraploid 'Northern Hi-Lights',
Tetraploid 'Tri-Lights'

Source: Kristian Theqvist

John and Sally Perkins

H: Wednesday, October 12, 2011

cumberlandense X arborescens is a Diploid

Rhododendron cumberlandense X Rhododendron arborescens has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron arborescens,
Diploid Rhododendron cumberlandense,
Diploid Rhododendron arborescens X Rhododendron cumberlandense,
Diploid Rhododendron cumberlandense X Rhododendron rborescens,
Diploid 'Pink Puff'

Source: George Hibben

John and Sally Perkins

H: Tuesday, October 11, 2011

arborescens X cumberlandense is a Diploid

Rhododendron arborescens X Rhododendron cumberlandense has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron arborescens,
Diploid Rhododendron cumberlandense,
Diploid Rhododendron arborescens X Rhododendron cumberlandense,
Diploid Rhododendron cumberlandense X Rhododendron rborescens,
Diploid 'Pink Puff'

Source: George Hibben

John and Sally Perkins

H: Wednesday, September 07, 2011

'Wisechap' is a Diploid

Rhododendron 'Wisechap', which is a Harry Wise hybrid documented as Rhododendron mucronulatum X Rhododendron minus var. chapmanii, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron minus,
Diploid Rhododendron minus var. chapmanii,
Diploid Rhododendron mucronulatum,
Diploid 'Wisechap'

Source: Doug Jolley

John and Sally Perkins

H: Monday, July 04, 2011

('Voluptuous' x 'Phipps Yellow') #3 is a Triploid

Rhododendron ('Voluptuous' x 'Phipps Yellow') #3, which is a Werner Brack hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid ('Voluptuous' x 'Phipps Yellow') #3, a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, ('Voluptuous' x 'Phipps Yellow') #3 having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Summary of Ploidy Levels

Diploid 'Phipps Yellow',
Diploid 'Voluptuous',
Triploid ('Voluptuous' x 'Phipps Yellow') #3

Source: Werner Brack

John and Sally Perkins

H: Monday, May 16, 2011

'Orchid Lights' is a Diploid

Rhododendron 'Orchid Lights', which is in the Northern Lights group from the University of Minnesota documented as Rhododendron canadense X Rhododendron x kosteranum, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron x kosteranum is documented as having been derived from Rhododendron molle.

Summary of Ploidy Levels

Diploid Rhododendron canadense
Diploid Rhododendron molle
Diploid Rhododendron 'Orchid Lights'
Tetraploid 'Apricot Surprise',
Tetraploid 'Candy Lights',
Tetraploid 'Golden Lights',
Tetraploid 'Lemon Lights',
Tetraploid 'Mandarin Lights',
Tetraploid 'Northern Hi-Lights',
Tetraploid 'Tri-Lights'

Source: John and Sally Perkins

John and Sally Perkins

H: Thursday, March 31, 2011

'Canobie Candy' X 'Hazel Hamilton' is a Diploid

One seedling of Rhododendron 'Canobie Candy' X Rhododendron flammeum 'Hazel Hamilton', which is Sally and John Perkins hybrid, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In this case, a diploid crossed with a diploid produced a diploid (26 chromosomes) indicating normal meiosis occurred where each diploid parent contributed a 1x gamete (13 chromosomes). Diploid X diploid yielding diploid is the expected outcome.

Summary of Ploidy Levels

Diploid Rhododendron canescens,
Diploid Rhododendron flammeum,
Diploid Rhododendron flammeum 'Hazel Hamilton',
Diploid 'Canobie Candy',
Diploid One seedling of 'Canobie Candy' X 'Hazel Hamilton'

Source: Joe Bruso

John and Sally Perkins

H: Saturday, January 08, 2011

'Markeeta’s Flame' is a Triploid

Rhododendron 'Markeeta’s Flame', which is Markeeta Nursery hybrid documented as 'Loderi Venus' x 'Anna', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Markeeta’s Flame', a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, 'Markeeta’s Flame' having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Rhododendron 'The Honorable Jean Marie de Montague' is known to throw unreduced gametes and Rhododendron 'Anna' has 'The Honorable Jean Marie de Montague' as a parent.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Loderi Venus',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Markeeta’s Flame',
Triploid 'Markeeta's Prize'

Source: Greer Gardens

John and Sally Perkins

H: Friday, January 07, 2011

'Markeeta's Prize' is a Triploid

Rhododendron 'Markeeta's Prize', which is Markeeta Nursery hybrid documented as 'Loderi Venus' x 'Anna', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of triploid 'Markeeta’s Prize', a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, 'Markeeta’s Prize' having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Rhododendron 'The Honorable Jean Marie de Montague' is known to throw unreduced gametes and Rhododendron 'Anna' has 'The Honorable Jean Marie de Montague' as a parent.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Loderi Venus',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Markeeta's Flame',
Triploid 'Markeeta's Prize'

Source: Greer Gardens

John and Sally Perkins

H: Saturday, December 11, 2010

'Taurus' is a Fertile Triploid

Rhododendron 'Taurus', which is a Frank Mossman hybrid documented as 'The Honourable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Frank Mossman considered Rhododendron 'Taurus' to be his one standout hybridizing achievement.

Rhododendron 'Taurus', although reluctant to be a parent, appears to be both seed and pollen fertile. Werner Brack raised several seedlings from open pollinated seed collected from a 'Taurus' in his garden. The 'Taurus' in the Brack garden was tested and shown to be a triploid. This provides direct evidence that a triploid Rhododendron can indeed set viable seed.

A seedling of a Jack Rosenthal cross of Rhododendron yakushimanum x 'Taurus' was, also, determined to be a triploid.

In the case of the triploid 'Taurus', a diploid crossed with a diploid produced a triploid indicating that unreduced meiosis most likely occurred for one of the diploid parents. In other words, 'Taurus' having 39 chromosomes most likely received 13 from one diploid parent and 26 chromosomes from the other diploid parent.

Rhododendron 'Taurus' was also determined to be a triploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Rhododendron 'Grace Seabrook', which is also documented as 'The Honorable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Summary of Ploidy Levels

Diploid 'The Honourable Jean Marie de Montague',
Triploid 'Grace Seabrook',
Triploid 'Taurus',
Triploid A seedling of Rhododendron yakushimanum x 'Taurus'

Source: Greer Gardens
Source: Werner Brack

John and Sally Perkins

H: Thursday, October 14, 2010

'Anita Gehnrich' is a Triploid

Rhododendron 'Anita Gehnrich', which is a Bud Gehnrich hybrid documented as 'The Honorable Jean Marie de Montague' X R. degronianum ssp. yakushimanum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Rhododendron 'Solidarity', which is documented as having the reversed parents of 'Anita Gehnrich', is also a triploid.

Summary of Ploidy levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Anita Gehnrich',
Triploid 'Solidarity'

Source: Werner Brack

John and Sally Perkins

H: Tuesday, October 12, 2010

'Solidarity' is a Fertile Triploid

Rhododendron 'Solidarity', which is a Hank Schannen hybrid documented as R. degronianum ssp. yakushimanum X 'The Honorable Jean Marie de Montague', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Solidarity' is known, on occasion, to produce open pollinated seedpods and this seed will sometimes germinate.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Rhododendron 'Anita Gehnrich', which is documented as the reverse cross of 'Solidarity, is also a triploid.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Anita Gehnrich',
Triploid 'Solidarity'

Source: Werner Brack

John and Sally Perkins

H: Sunday, October 10, 2010

'Grace Seabrook' is a Triploid

Rhododendron 'Grace Seabrook', which is a Cecil Seabrook hybrid documented as 'The Honorable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Taurus', which is also documented as 'The Honorable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch. 'Taurus', although reluctant to be a parent, appears to be both seed and pollen fertile.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Grace Seabrook',
Triploid 'Taurus'

Source: Werner Brack

John and Sally Perkins
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