'Princesse d' Orange' is a Tetraploid

Rhododendron 'Princesse d' Orange', which is a Ghent hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Miniatum Floribundum',
Triploid 'Aida',
Triploid 'Bronze Unique',
Triploid 'Croceum Tricolor',
Triploid 'Daviesii',
Triploid 'Deep Rose',
Triploid 'Freya',
Triploid 'Gloria Mundi',
Triploid 'Il Tasso',
Triploid 'Josephine Klinger',
Triploid 'Phebe',
Triploid 'Phidias',
Triploid 'Pontica Album',
Triploid 'Van Houttei Flore Pleno',
Triploid 'Velasquez',
Triploid/Tetraploid 'Praxitèle',
Tetraploid 'Admiraal De Ruyter',
Tetraploid 'Altaclerensis',
Tetraploid 'Ariel',
Tetraploid 'Beaté Céleste',
Tetraploid 'Bijou des Amateurs',
Tetraploid 'Bouquet de Flore',
Tetraploid 'Cardinal,
Tetraploid 'Comte de Flandre',
Tetraploid 'Corneille',
Tetraploid 'Directeur Charles Baumann',
Tetraploid 'Durante Alle',
Tetraploid 'Electum',
Tetraploid 'Eugénie',
Tetraploid 'Fénelon',
Tetraploid 'Flameola Incarnata',
Tetraploid 'Héroine Plena,
Tetraploid 'Heureuse Surprise',
Tetraploid 'Hollandia',
Tetraploid 'Hora',
Tetraploid 'Ignacea',
Tetraploid 'Igneum Novum',
Tetraploid 'Il Tasso',
Tetraploid 'Jenny Lind',
Tetraploid 'Juliana Nova',
Tetraploid 'Julius Caesar',
Tetraploid 'Leibuir',
Tetraploid 'Lelia',
Tetraploid 'Madame Gustave Guillemot',
Tetraploid 'Marie Verschaffelt',
Tetraploid 'Minerva',
Tetraploid 'Nancy Waterer',
Tetraploid 'Oscar I',
Tetraploid 'Perfecta',
Tetraploid 'Princesse d' Orange',
Tetraploid 'Raphael de Smet'

Source: Azaleatuin

John and Sally Perkins

atlanticum X caledulaceum is a Tetraploid

[Rhododendron atlanticum X Rhododendron calendulaceum] "Broken Arrow", which is a Dick Jaynes hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Tetraploid Rhododendron atlanticum,
Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron atlanticum X Rhododendron calendulaceum "Broken Arrow",
Tetraploid Rhododendron atlanticum X Rhododendron calendulaceum orange,
Tetraploid Rhododendron atlanticum X Rhododendron calendulaceum pink

Source: Arnold Arboretum Accession Number: 410-2002

John and Sally Perkins

'Wingard's Gold' is a Tetraploid

Rhododendron 'Wingard's Gold' has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Tetraploid 'Wingard's Gold'

Source: Mike Creel

John and Sally Perkins

Rhododendron luteum is a Tetraploid

Rhododendron luteum has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron luteum was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Tetraploid Rhododendron luteum,
Tetraploid Rhododendron luteum 'Golden Comet',
Tetraploid Rhododendron luteum Orange form,
Tetraploid Rhododendron luteum Yellow form

Source: Arnold Arboretum
Source: John and Sally Perkins
Source: Kristian Theqvist

John and Sally Perkins

"Orange Ruffle" is a Tetraploid

Rhododendron "Orange Ruffle", which is a Frank Abbott hybrid, has been determined to be a tretraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Tetraploid Rhododendron ""Orange Ruffle"

Source: John Abbott

John and Sally Perkins

Weston Nurseries Plants Still Available

We still have some of the Weston Nurseries hybrids available for sale.

 

In keeping with our Donated Plant sale tradition whereby at the end of the sale we cut all prices in half, prices on these plants will remain at half price, as follows.

 

Prices

$10 per 6-pack. 

$22 per Flat (three 6-packs)

 

All proceeds go to the Mass Chapter. These are all excellent plants, and do not look like "left overs". 

If you need to create a hedge, or want to procure plants for growing on as gifts, this is a low-cost way to do so.

 

The plants are at my home in Hopkinton, MA.  Contact me to arrange a time to stop by to make a purchase.

 

Selections

Cultivar	Rhodo/Azalea Type	Description	Zone
smirnowii x yak	Broad leaved evergreen Rhodo	Pink, fading to lighter pink or white.  Deep green leaves with heavy indumentum.	5
Blue Baron	Small leaved evergreen Rhodo (lep)	Bluest of blue flowers.	6
Milestone	Small leaved evergreen Rhodo (lep)	Dark Pink (red if you use your imagination).  Blooms in April.	5
Abigail Adams	Evergreen Azalea	Large pink blooms.  Wider than tall.  Does well in heavy shade or sun	5
Millennium	Deciduous Azalea	Fragrant, red, in mid-July.  Leaves with silver/green undersides.	4
Nectar	Deciduous Azalea	Fragrant, pink-orange, in late June	5

 

 

Joe Bruso

61 South Mill St.

Hopkinton, MA

jpbruso@aol.com

508.435.8217

"Graftonense" is a Tetraploid

Rhododendron "Graftonense", which is a Frank Abbott hybrid, has been determined to be a tretraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Tetraploid Rhododendron "Graftonense"

Source: John Abbott

John and Sally Perkins

"Gladywense" is a Triploid

Rhododendron "Gladywense" has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Triploid Rhododendron "Gladywense"

Source: Arnold Arboretum Accession Number: 979-67-A

John and Sally Perkins

Rhododendron "calendulaceum not" is a Triploid

Rhododendron "calendulaceum not", which appears to be a hybrid between Rhododendron flammeum and Rhododendron calendualceum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron flammeum,
Triploid Rhododendron "calendulaceum not",
Tetraploid Rhododendron calendualceum

Source: Arnold Arboretum Accession Number: 109-2007-Y

John and Sally Perkins

'Unique' is a Triploid?

Rhododendron 'Unique', which is a Ghent hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Unique' was determined to be a tetraploid using flow cytometry by Tom Eeckhaut.

Summary of Ploidy Levels

Triploid 'Unique'

Source: Arnold Arboretum Accession Number: 72-57-B

John and Sally Perkins

Clones may not be Identical

"Using DNA sequencing techniques that can decode the complete genome of an organism in one go (so-called ‘whole genome sequencing’) the researchers analyzed ‘clones’ of the small flowering plant ‘thalecress’ (Arabidopsis). They found that observable variations in regenerant plants are substantially due to high frequencies of mutations in the DNA sequence of these regenerants, mutations which are not contained in the genome of the parent plant."

Why plant 'clones' aren't identical

Rhododendron occidentale hybrid is a Triploid

A Rhododendron occidentale hybrid has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron occidentale,
Triploid Rhododendron occidentale hybrid

Source: George Hibben

John and Sally Perkins

Audra State Park contains Triploids

Two deciduous azalea hybrids in Audra State Park, West Virginia have been determined to be triploids using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Audra State Park in West Virginia contains Rhododendron arborescens, Rhododendron maximum, Rhododendron periclymenoides, maybe
Rhododendron prinophyllum, and Rhododendron calendulaceum.

Summary of Ploidy Levels

Diploid Rhododendron arborescens,
Diploid Rhododendron maximum,
Diploid Rhododendron periclymenoides,
Diploid Rhododendron prinophyllum,
Triploid 2 deciduous azalea hybrids in Audra State Park,
Tetraploid Rhododendron calendulaceum

Source: Audra State Park, West Virginia

John and Sally Perkins

Reminder: Massachusetts Chapter Meeting @ Wed Sep 21 6:30pm - 9pm (ARS Massachusetts Chapter Calendar)

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"Pastel #20" is a Diploid

"Pastel #20" has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

The Pastel series is documented to contain Rhododendron alabamense,
Rhododendron canescens, and Rhododendron flammeum.

Summary of Ploidy Levels

Diploid Rhododendron alabamense,
Diploid Rhododendron canescens,
Diploid Rhododendron flammeum,
Diploid 'Casille',
Diploid "Pastel #6",
Diploid "Pastel #7",
Diploid "Pastel #20"

Source: Woodlanders Nursery

John and Sally Perkins

Donated Plant Sale - Seedlings

In additional to the Weston plants, 6 inch tall deciduous azalea seedlings from a cross of a "White Calendulaceum" Skinner 166 by calenducaleum 'Smokey Mountaineer' will be sold at our annual Donated Plant Sale, occurring at our next Chapter meeting, Wednesday, September 21 at the Elm Bank East Wing.

The cost of these deciduous azalea seedlings will be 1 for $5 or 3 for $10. These seedlings could range in color from white to pink to yellow to orange to red.

"Pastel #7" is a Diploid

"Pastel #7" has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

The Pastel series is documented to contain Rhododendron alabamense,
Rhododendron canescens, and Rhododendron flammeum.

Summary of Ploidy Levels

Diploid Rhododendron alabamense,
Diploid Rhododendron canescens,
Diploid Rhododendron flammeum,
Diploid 'Casille',
Diploid "Pastel #6",
Diploid "Pastel #7",
Diploid "Pastel #20"

Source: Woodlanders Nursery

John and Sally Perkins

"Pastel #6" is a Diploid

"Pastel #6" has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

The Pastel series is documented to contain Rhododendron alabamense,
Rhododendron canescens, and Rhododendron flammeum.

Summary of Ploidy Levels

Diploid Rhododendron alabamense,
Diploid Rhododendron canescens,
Diploid Rhododendron flammeum,
Diploid 'Casille',
Diploid "Pastel #6",
Diploid "Pastel #7",
Diploid "Pastel #20"

Source: Woodlanders Nursery

John and Sally PerkinsDiploid 'Casille',

'Phyllis Korn' x 'Trude Webster' is a Pentaploid

Two seedlings of Rhododendron 'Phyllis Korn' x 'Trude Webster', which is a Jim Barlup hybrid, have been determined to be a pentaploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the pentaploid seedlings of 'Phyllis Korn' (3X) x 'Trude Webster' (4X), a triploid by a tetraploid produced a pentaploid indicating that the triploid seed parent 'Phyllis Korn' was most likely unreduced. In other words, the pentaploid seedlings of 'Phyllis Korn' x 'Trude Webster' having 65 chromosomes most likely received 39 chromosomes from the triploid 'Phyllis Korn' and 26 chromosomes from the tetraploid 'Trude Webster'. Considering the parents are a triploid and a neotetraploid, meiosis may not be normal for either parent.

Summary of Ploidy Levels

Triploid 'Phyllis Korn'
Tetraploid 'Trude Webster',
Pentaploid 2 Seedlings of 'Phyllis Korn' x 'Trude Webster'

Source: Jim Barlap

John and Sally Perkins

Special Sale of Weston Nurseries Plants

To Mass Chapter Members,

 

As occurred 2 years ago, we again have the opportunity to purchase very hardy Weston Nurseries-developed rhododendrons and azaleas at irresistible prices.  Wayne Mezitt, owner of Weston Nurseries, has provided the Chapter with small plants in order to get them into the hands of people who can appreciate them.  Since Weston Nurseries has stopped doing their own propagation, these are likely to be the last small plants we will obtain from them.  This is an opportunity to obtain some of Weston's best introductions at great prices.  These plants were bred for the New England climate and should perform exceptionally well for you.

 

Supplies for some selections should be sufficient, but others are limited.  Most are in 3" cells, with a few in 2" cells.  In many cases there are two plants to the cell, which can be separated.  Many of the deciduous azaleas have bloom buds.

 

The plants will be sold at our annual Donated Plant Sale, occurring at our next Chapter meeting, Wednesday, September 21 at the Elm Bank East Wing.  See the latest Chapter Newsletter for directions and details on the events scheduled for this meeting.  Due to the volume of plants available, sales will begin about 6:30 PM, prior to the start of the Pot Luck Supper.  Please arrive early for the best selection.

 

Prices

$20 per 6-pack. 

$45 for three 6-packs (1 flat in most cases). Each 6-pack contains 6 to 10 plants.

Exceptions: 

·         Smirnowii-Yak hybrid, in individual 3" pots, are $3 each.

·         Selections in short supply (as noted below) will be sold as 3 packs at $9 each to allow more people to obtain them.

 

 

Selections

Cultivar	Rhodo/Azalea Type	Description	Limited Supply?
smirnowii x yak	Broad leaved evergreen Rhodo	Pink, fading to lighter pink or white.  Deep green leaves with heavy indumentum.
Blue Baron	Small leaved evergreen Rhodo (lep)	Bluest of blue flowers.	Y
Milestone	Small leaved evergreen Rhodo (lep)	Dark Pink (red if you use your imagination).  Blooms in April.	Y
Abigail Adams	Evergreen Azalea	Large pink blooms.  Wider than tall.  Does well in heavy shade or sun
R. kiusianum	Evergreen Azalea	Tiny leaves.  One of best evergreen azalea species for New England.	Y
Anna's Smile	Deciduous Azalea	Fragrant, pink with golden eye turning peachy pink, in early June.	Y
Golden Showers	Deciduous Azalea	Fragrant, peach/orange, in mid-July.	Y
Millennium	Deciduous Azalea	Fragrant, red, in mid-July.  Leaves with silver/green undersides.
Nectar	Deciduous Azalea	Fragrant, pink-orange, in late June

 

A few words on some selections:

 

I consider Abigail Adams the best hybrid evergreen azalea for my Zone 5b garden.  Unlike many evergreen azalea hybrids, it is fully hardy for me, never showing any winter damage.  The very large blooms are a wonderful shade of medium pink, totally covering the plant with, even in a shady location. When grown in adequate sun, the foliage turns orange/red in the fall.

 

Anyone who has seen Blue Barron in bloom in our display garden knows what a special plant it is.  It is one of, if not the bluest, most floriferous of the blue small leaved rhododendrons that can be grown in our area.  Blue Barron won Best in Show in this year's Truss Show.  Need I say more?  We have a limited supply of this cultivar.  First come, first served.

 

Joe Bruso

Rhododendron diaprepes X sutchuenense is a Diploid

Rhododendron diaprepes X Rhododendron sutchuenense has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron diaprepes 'Gargantua', which is a tetraploid, may have been one of the parents in the cross of Rhododendron diaprepes X Rhododendron sutchuenense. Neotetraploids can produce diploid offspring.

Rhododendron diaprepes is most likely a diploid species.

Summary of Ploidy Levels

Diploid Rhododendron diaprepes X Rhododendron sutchuenense,
Tetraploid Rhododendron diaprepes 'Gargantua'

Source: Al Fitzburg

John and Sally Perkins

Reminder: Massachusetts Chapter Meeting @ Wed Sep 21 6:30pm - 9pm (John Perkins)

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'English Roseum' X [diaprepes X hyperthrum] is a Diploid

Rhododendron 'English Roseum' X [Rhododendron diaprepes X Rhododendron hyperthrum], which is a Thornton hybrid, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron diaprepes 'Gargantua', which is a tetraploid, may have been one of the parents in the cross of Rhododendron diaprepes X Rhododendron hyperthrum. Neotetraploids and triploids can produce diploid offspring.

Rhododendron diaprepes is most likely a diploid species.

Summary of Ploidy Levels

Diploid Rhododendron 'English Roseum' X [Rhododendron diaprepes X Rhododendron hyperthrum],
Tetraploid Rhododendron diaprepes 'Gargantua'

Source: John Thornton

John and Sally Perkins

Fertile Triploid Orchids

Dactylorhiza fuchsii x purpurella (D. x venusta)

"This tuberous perennial herb is frequent wherever the parents grow together, such as in marshes, fens and wet meadows. It is especially frequent in man-made habitats such as quarries and industrial waste land. It may persist in the absence of D. purpurella if drainage has caused the loss of this species from the site. This partially fertile triploid hybrid can back-cross with its parents, and is often present in long-lived hybrid swarms."

Dactylorhiza fuchsii is a diploid species.

Dactylorhiza purpurella is a tetraploid species.

http://www.brc.ac.uk/plantatlas/index.php?q=list_plants&page=81

'Sham's Ruby' X 'J.L. Creech' is a Diploid

Rhododendron 'Sham's Ruby' X 'J.L. Creech', which is a Thornton hybrid, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Sham's Ruby' X 'J.L. Creech'

Source: John Thornton

John and Sally Perkins

Reminder: Board Meeting @ Wed Sep 14 7:30pm - 9pm (John Perkins)

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'Bibiani' X 'J.L. Creech' is a Diploid

Rhododendron 'Bibiani' X 'J.L. Creech', which is a Thornton hybrid, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Bibiani',
Diploid 'Bibiani' X 'J.L. Creech'

Source: John Thornton

John and Sally Perkins

'Fox's Favorite' is a Triploid

Rhododendron 'Fox's Favorite' has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Fox's Favorite', which is a red, is suspected of being either 'Grace Seabrook' or 'Taurus'. The ploidy results support this position.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Fox's Favorite',
Triploid 'Grace Seabrook',
Triploid 'Taurus'

Source: Bob Fox

John and Sally Perkins

"Looye's Blue" is a Tetraploid

Rhododendron "Looye's Blue", which is a Jack and Jackie Looye hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Tetraploid Looye's Blue"

Source: Rarefind Nursery

John and Sally Perkins

'Northern Starburst' is a Tetraploid

Rhododendron 'Northern Starburst', which is a Brigg's chemically treated 'PJM', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron dauricum,
Diploid Rhododendron minus,
Tetraploid 'Northern Starburst'

Source: Rarefind Nursery

John and Sally Perkins

keiskei X (spinuliferum X keiskei) is a Diploid

Rhododendron keiskei X (Rhododendron spinuliferum X Rhododendron keiskei) has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron keiskei,
Diploid Rhododendron keiskei X (Rhododendron spinuliferum X Rhododendron keiskei)

Source: Rarefind Nursery

John and Sally Perkins

Reminder: Board Meeting @ Wed Sep 14 7:30pm - 9pm (John Perkins)

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Polyploidy and Orchids

A Brief Look at Polyploidy in Orchids by Paul Gripp

Notice triploid orchids can be fertile.

Notice triploid orchids usually look more like the tetraploid parent.

'Wisechap' is a Diploid

Rhododendron 'Wisechap', which is a Harry Wise hybrid documented as Rhododendron mucronulatum X Rhododendron minus var. chapmanii, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca Oliveira, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron minus,
Diploid Rhododendron minus var. chapmanii,
Diploid Rhododendron mucronulatum,
Diploid 'Wisechap'

Source: Doug Jolley

John and Sally Perkins

'Minerva' is a Tetraploid

Rhododendron 'Minerva', which is a Ghent hybrid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Miniatum Floribundum',
Triploid 'Aida',
Triploid 'Bronze Unique',
Triploid 'Croceum Tricolor',
Triploid 'Daviesii',
Triploid 'Deep Rose',
Triploid 'Freya',
Triploid 'Gloria Mundi',
Triploid 'Il Tasso',
Triploid 'Josephine Klinger',
Triploid 'Phebe',
Triploid 'Phidias',
Triploid 'Pontica Album',
Triploid 'Van Houttei Flore Pleno',
Triploid 'Velasquez',
Tetraploid 'Admiraal De Ruyter',
Tetraploid 'Altaclerensis',
Tetraploid 'Ariel',
Tetraploid 'Beaté Céleste',
Tetraploid 'Bijou des Amateurs',
Tetraploid 'Bouquet de Flore',
Tetraploid 'Cardinal,
Tetraploid 'Comte de Flandre',
Tetraploid 'Corneille',
Tetraploid 'Directeur Charles Baumann',
Tetraploid 'Durante Alle',
Tetraploid 'Electum',
Tetraploid 'Eugénie',
Tetraploid 'Fénelon',
Tetraploid 'Flameola Incarnata',
Tetraploid 'Héroine Plena,
Tetraploid 'Heureuse Surprise',
Tetraploid 'Hollandia',
Tetraploid 'Hora',
Tetraploid 'Ignacea',
Tetraploid 'Igneum Novum',
Tetraploid 'Il Tasso',
Tetraploid 'Jenny Lind',
Tetraploid 'Juliana Nova',
Tetraploid 'Julius Caesar',
Tetraploid 'Leibuir',
Tetraploid 'Lelia',
Tetraploid 'Madame Gustave Guillemot',
Tetraploid 'Marie Verschaffelt',
Tetraploid 'Minerva',
Tetraploid 'Nancy Waterer',
Tetraploid 'Oscar I',
Tetraploid 'Perfecta',
Tetraploid 'Raphael de Smet'

Source: Azaleatuin

John and Sally Perkins

September Newsletter

Rhododendron arborescens X Rhododendron calendulaceum is a Triploid

One seedling of Rhododendron arborescens X Rhododendron calendulaceum has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron arborescens is diploid species. Rhododendron calendulaceum is a tetraploid species.

In the case of these triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron arborescens,
Triploid 5 seedlings from Rhododendron occidentale SM189 X Rhododendron calendulaceum 'Colossus',
Tetraploid Rhododendron calendulaceum

Source: Bruce Clyburn

John and Sally Perkins

occidentale SM189 X calendulaceum 'Colossus' is a Triploid

Five seedlings of Rhododendron occidentale SM189 X Rhododendron calendulaceum 'Colossus' have been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron occidentale is diploid species. Rhododendron calendulaceum is a tetraploid species.

In the case of these 5 triploids (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron occidentale,
Triploid 5 seedlings from Rhododendron occidentale SM189 X Rhododendron calendulaceum 'Colossus',
Tetraploid Rhododendron calendulaceum

Source: Bruce Clyburn

John and Sally Perkins

Rhododendron viscosum forma rhodanthum is a Diploid

Rhododendron viscosum forma rhodanthum has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron viscosum forma rhodanthum appears to be a natural hybrid most likely containing Rhododendron cumberlandense.

Rhododendron viscosum was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron cumberlandense,
Diploid Rhododendron viscosum,
Diploid Rhododendron viscosum forma rhodanthum,
Diploid Rhododendron viscosum var. serrulatum

Source: Arnold Arboretum Accession Number: 638-62

John and Sally Perkins

Rhododendron viscosum var. serrulatum is a Diploid

Rhododendron viscosum var. serrulatum has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron viscosum was also determined to be a diploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Diploid Rhododendron viscosum,
Diploid Rhododendron viscosum forma rhodanthum,
Diploid Rhododendron viscosum var. serrulatum

Source: Connecticut College Arboretum Accession Number: 90-7-A

John and Sally Perkins

Rhododendron calendulaceum var. croceum is a Tetraploid

Rhododendron calendulaceum var. croceum has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron calendulaceum was also determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

Summary of Ploidy Levels

Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron calendulaceum var. croceum,
Tetraploid Rhododendron calendulaceum 'Cherokee',
Tetraploid Rhododendron calendulaceum 'Smoky Mountaineer'

Source: Arnold Arboretum Accession Number: 17758-1-A

John and Sally Perkins