ARS Mass Chapter Founders' Day Brunch November 14

As a reminder, in 2 weeks we will have our annual Founders' Day Brunch.

Our guest speaker will be Dick Jaynes, the foremost expert on the genus Kalmia (Mountain Laurels)

We will also have our Seed Exchange at this meeting.

 

Location this year is at Pappa Razzi in Wellesley (directions are in the most recent Newsletter).

Cost:  $30 per person.

 

Please contact our treasurer Pete Littlefield to reserve your spot at this event.

Pete's contact info is:

75 Oak St.

Holliston, MA  01764

508.429.6470  or woodland.gardener@verizon.net

 

Joe Bruso

Rhododendron maximum 'Kalamity' is a Diploid

Rhododendron maximum 'Kalamity' has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron maximum 'Kalamity', which was found growing native in North Carolina, is a maximum that by initial appearances could pass for a Kalmia latifolia. At the Arnold Arboretum, 'Kalamity' is planted among the Kalmia latifolia and blends in so well most visitors to the Arnold fail to notice it despite being planted in the front row along a major pathway.

Rhododendron maximum 'Kalamity' was originally documented as a cross between Rhododendron maximum X Kalmia latifolia. However DNA research by Grant, Toomey, and Culham has clearly shown that 'Kalamity' is merely an odd looking Rhododendron maximum having the "normal" DNA associated with the species Rhododendron maximum.

'Kalmia X Rhododendron is a Myth'

'Kalmia X Rhododendron Debunked'

Summary of Ploidy Levels

Diploid Rhododendron maximum 'Kalamity'

Source: Arnold Arboretum Accession Number: 194-82-A

John and Sally Perkins

"Cahas Mountain White" is a Tetraploid

Rhododendron calendulaceum "Cahas Mountain White" has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

A population of Rhododendron calendulaceum exists on Cahas Mountain in southern Virginia that display typical calendulaceum characteristics except for having flower colors that are more pastel than those normally associated with calendulaceum.

Summary of Ploidy Levels

Tetraploid Rhododendron calendulaceum "Cahas Mountain White"

Source: John and Sally Perkins

John and Sally Perkins

'Mrs A.T. de la Mare' is a Diploid

Rhododendron 'Mrs. A.T. de la Mare', which is a Van Nes hybrid documented as R. fortunei 'Sir Charles Butler' x 'Halopeanum' has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Halopeanum'
Diploid 'Mrs A.T. de la Mare'

Source: John and Sally Perkins
Source: John Thornton

John and Sally Perkins

'Chattooga' is a Tetraploid

Rhododendron 'Chattooga' has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Chattooga', found near the Chattooga River which forms the boundary between South Carolina and Georgia, is a natural hybrid of Rhododendron calendulaceum. 'Chattooga' is both seed and pollen fertile.

Audra State Park in West Virginia also contains natural hybrids of Rhododendron calendulaceum. Such natural hybrids occur when tetraploid and diploid deciduous azalea species, blooming at the same time, coexist.

Rhododendron calendulaceum is a tetraploid species.

Summary of Ploidy levels

Tetraploid 'Chattooga',
Tetraploid Rhododendron calendulaceum

Source: John and Sally Perkins

John and Sally Perkins

Rhododendron calendulaceum 'Cherokee' is a Tetraploid

Rhododendron calendulaceum 'Cherokee' has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group
Centre for Functional Ecology
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra.

John and Sally Perkins used Rhododendron calendulaceum 'Cherokee' as the seed parent in testing their conjecture that tetraploid deciduous azalea species normally reject pollen from diploid deciduous azaleas. Their test indicated that in all 5 cases where tetraploid pollen was placed on 'Cherokee' large seedpods were produced whereas in all 13 cases where diploid was placed on 'Cherokee' no seedpods were produced. This result was completely consistent with results the Perkins had experienced previously when working with other tetraploid deciduous azaleas as seed parents.

John and Sally Perkins have shown that diploid deciduous azalea do accept pollen from both diploid and tetraploid deciduous azaleas.

Rules of Engagement

Summary of Ploidy Levels

Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron calendulaceum var. croceum,
Tetraploid Rhododendron calendulaceum 'Cherokee',
Tetraploid Rhododendron calendulaceum 'Smoky Mountaineer'

Source: John and Sally Perkins

John and Sally Perkins

'Lady Eleanor Cathcart' is a Diploid

Rhododendron 'Lady Eleanor Cathcart', which is a 1850 John Waterer hybrid documented as R. maximum x R. arboreum, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Lady Eleanor Cathcart'

Source: Jane Brooks

John and Sally Perkins

'Duke of York' is a Diploid

Rhododendron 'Duke Of York', which is a Paul hybrid documented as R. fortunei ssp. fortunei x 'Scipio', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Duke Of York'

Source: Arnold Arboretum Accession Number: 17601

John and Sally Perkins

'Trude Webster' is a Tetraploid

Rhododendron 'Trude Webster', which is a Harold Greer hybrid documented as 'Countess of Derby' X 'Countess of Derby', has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

The hybridization work of Jim Barlup indicates that 'Trude Webster' is fertile in both directions when crossed with known and suspected polyploid elepidotes.

'Countess of Derby', which was determined to be a tetraploid, is the documented pollen parent of 'Anna Rose Whitney', which has been determined to be a triploid by the Coimbra based research team.

'Pink Pearl', which is the documented seed parent of 'Countess of Derby', was also determined to be a triploid by the Coimbra based research team.

'Cynthia', which is the documented pollen parent of 'Countess of Derby', was also determined to be a triploid by the Coimbra based research team.

The findings of the Coimbra based research team indicate 6 multi generational polyploid branches starting with the triploid 'Broughtonii', namely 1)'Broughtonii', 'Pink Pearl', 'Gomer Waterer', and 'Phyllis Korn', 2)'Broughtonii', 'Pink Pearl', 'Cynthia', 'Countess of Derby', and 'Anna Rose Whitney', 3)'Broughtonii', 'Pink Pearl', 'Cynthia', 'Countess of Derby', and 'Van',
4)'Broughtonii', 'Pink Pearl', 'Cynthia', 'Countess of Derby', and 'Lucky Strike',
5)'Broughtonii', 'Pink Pearl', 'Cynthia', 'Countess of Derby', 'Trude Webster', 'Platinum Pearl', and 'Gentle Giant' and 6)'Broughtonii', 'Pink Pearl', 'Antoon van Welie', 'Lydia', 'Trude Webster', and 'Grand Slam'.

Summary of Ploidy Levels

Triploid 'Anna Rose Whitney',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Gomer Waterer',
Triploid 'Lydia',
Triploid 'Lucky Strike',
Triploid 'Pink Pearl',
Triploid 'Phyllis Korn',
Triploid 'Platinum Pearl',
Triploid 'Van',
Tetraploid 'Antoon van Welie',
Tetraploid 'Countess of Derby',
Tetraploid 'Gentle Giant',
Tetraploid 'Grand Slam',
Tetraploid 'Trude Webster'

Source: Greer Gardens
Source: Bob Fox

John and Sally Perkins

'Josephine Klinger' is a Triploid

Rhododendron 'Josephine Klinger', which is a 1869 J. Baumann Ghent hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Josephine Klinger' appears to be sterile as both a seed and pollen parent indicating that it may represent a first generation cross of a diploid and tetraploid deciduous azalea species. The Ghents are documented as having been produced from crossing diploid and tetraploid deciduous azalea species. This result appears to support that documentation.

Summary of Ploidy Levels

Triploid 'Bronze Unique',
Triploid 'Daviesii',
Triploid 'Deep Rose',
Triploid 'Gloria Mundi',
Triploid 'Josephine Klinger'

Source: John and Sally Perkins

John and Sally Perkins

'Gartendirektor Rieger' is a Triploid

Rhododendron 'Gartendirektor Rieger', which is a Hobbie hybrid documented as 'Adriaan Koster' x R. williamsianum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group
Centre for Functional Ecology
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Triploid 'Gartendirektor Rieger'

Source: Jane Brooks

John and Sally Perkins

'Anna' is a Diploid

Rhododendron 'Anna', which is a Lem hybrid documented as 'Norman Gill' x 'The Honorable Jean Marie de Montague', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Anna' is both seed and pollen fertile.

'Anna' is the seed parent of 'Lem's Monarch' and 'Point Defiance', which were determined to be a tetraploids using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch. This may indicate that 'Anna' occasionally throws unreduced gametes.

'Anna' is the pollen parent of 'Lem's Cameo', which was also determined to be a diploid by the Coimbra based research team.

'The Honorable Jean Marie de Montague', which is the pollen parent of 'Anna', is suspected of throwing more unreduced gametes than is normal for a diploid elepidote.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Lem's Cameo',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Markeeta’s Flame',
Triploid 'Markeeta’s Prize'

Source: Whitney Nursery

John and Sally Perkins

'Nancy Evans' is a Diploid

Rhododendron 'Nancy Evans', which is a Ned Brockenbrough hybrid documented as 'Hotei' x 'Lem's Cameo', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

This Coimbra based research team also determined that 'Hotei' and 'Lem's Cameo' are diploids.

Rhododendron 'Nancy Evans' is seed fertile but pollen sterile.

'Nancy Evans' is the seed parent of 'Horizon Monarch', which was determined to be a tetraploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch. This indicates that 'Nancy Evans' may occasionally throw unreduced gametes.

Summary of Ploidy Levels

Diploid 'Hotei',
Diploid 'Lem's Cameo',
Diploid 'Nancy Evans',
Tetraploid 'Horizon Monarch'

Source: Whitney Nursery

John and Sally Perkins

Wednesday Chapter Meeting

Tomorrow, Wednesday October 20 in the East Wing at Elm Bank in Wellesley

Jeanie Gillis of the Heritage Plantation will talk.  We will have our Fall Foliage Show so bring your best and worst terminals for registration at 7:00 PM.  Bring a few select cuttings in plastic bags, well labeled for our cutting exchange after the talk and show.  Members with names starting with A-M  bring munchies to share.

'Lem's Cameo' is a Diploid

Rhododendron 'Lem's Cameo', which is a Halfdan Lem hybrid documented as 'Dido Group' x 'Anna', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Lem's Cameo' is fertile as both a seed and pollen parent.

Summary of Ploidy Levels

Diploid 'Anna',
Diploid 'Lem's Cameo',
Diploid 'Norman Gill',
Diploid 'The Honorable Jean Marie de Montague'

Source Marc Colombel

John and Sally Perkins

Reminder: Massachusetts Chapter Meeting @ Wed Oct 20 7:30pm - 9:30pm (ARS Massachusetts Chapter Calendar)

more details » Massachusetts Chapter Meeting Meeting When Wed Oct 20 7:30pm – 9:30pm Eastern Time Where Elm Bank Wellsley, MA (map) Calendar ARS Massachusetts Chapter Calendar Who • John Perkins - creator

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'Anna Rose Whitney' is a Fertile Triploid

Rhododendron 'Anna Rose Whitney', which is van Veen hybrid introduced by William Whitney and documented as R. griersonianum x 'Countess of Derby', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Anna Rose Whitney', which has 'Broughtonii', 'Pink Pearl', and 'Countess of Derby'
as ancestors, is the pollen parent of 'Hurricane'; however, like many (semi)fertile triploids 'Anna Rose Whitney' is reluctant to produce viable seedlings as either a seed or pollen parent.

In the case of triploid 'Anna Rose Whitney', a suspected diploid crossed with a tetraploid produced a triploid indicating that normal reduced meiosis most likely occurred for both parents. In other words, 'Anna Rose Whitney' having 39 chromosomes received 13 chromosomes from the suspected diploid R. griersonianum and 26 chromosomes from the tetraploid 'Countess of Derby'.

Summary of Ploidy Levels

Diploid 'Hurricane',
Triploid 'Broughtonii',
Triploid 'Pink Pearl',
Triploid 'Anna Rose Whitney',
Tetraploid 'Countess of Derby'

Source: Whitney Nursery

John and Sally Perkins

'Pink Pearl' is a Fertile Triploid.

Rhododendron 'Pink Pearl', which is a Waterer hybrid documented as 'George Hardy' x 'Broughtonii', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group
Centre for Functional Ecology
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra.

'Pink Pearl' is sometimes referred to as the first commercially successful Rhododendron.

Other flow cytometry results from this research team include the following

'Broughtonii', the documented pollen parent of 'Pink Pearl', is a triploid.

'Antoon van Welie', which has 'Pink Pearl' as a documented seed parent, is a tetraploid.

'Gomer Waterer', which has 'Pink Pearl' as a documented pollen parent, is a
triploid.

'Graf Zeppelin', which has 'Pink Pearl' as a documented seed parent, is a
diploid.

'J.G. Millais', which has 'Pink Pearl' as a documented pollen parent, is a diploid.

'Topsvoort Pearl', which is documented sport of 'Pink Pearl', is a triploid.

'Ebony Pearl', which is documented sport of 'Pink Pearl', is a triploid.

'Mother of Pearl', which is a documented sport of 'Pink Pearl', is a diploid.

'Phyllis Korn', which has 'Gomer Waterer' as a document pollen parent, is a triploid.

'Countess of Derby', which has 'Pink Pearl' as the documented seed parent and 'Cynthia' as the documented pollen parent, is a tetraploid.

'Trude Webster', which is documented as 'Countess of Derby' selfed, is a tetraploid.

'Diane', which is documented as the seed parent of 'Phyllis Korn', is a diploid.

'Summer Peach', which has 'Phyllis Korn' as a documented pollen parent, is a diploid.

'Summer Wind' and 'Peach Recital', which have 'Summer Peach' as a documented pollen parent, are a diploids.

Seedlings of Jim Barlup's cross of 'Phyllis Korn' by 'Trude Webster' have been determined to be pentaploids.

These results from the researchers at the University of Coimbra indicate 4 generations of fertile triploids, that triploids can produce diploid, triploid, tetraploid, and pentaploid offspring, and sports of triploids can be both ploidy stable and ploidy unstable.

Summary of Ploidy Levels

Diploid 'Diane',
Diploid 'Graf Zeppelin',
Diploid 'J.G. Millais',
Diploid 'Mother of Pearl',
Diploid 'Peach Recital,
Diploid 'Summer Peach',
Diploid 'Summer Wind',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Ebony Pearl',
Triploid 'Gomer Waterer',
Triploid 'Phyllis Korn',
Triploid 'Pink Pearl',
Triploid 'Topsvoort Pearl',
Tetraploid 'Antoon van Welie',
Tetraploid 'Countess of Derby',
Tetraploid 'Trude Webster',
Pentaploid Seedlings of 'Phyllis Korn' X 'Trude Webster'

Source: Whitney Nursery

John and Sally Perkins

Reminder: P4M Workday: Planeview Nursery - Pot Cuttings @ Sat Oct 23 10am - 12pm (ARS Massachusetts Chapter Calendar)

more details » P4M Workday: Planeview Nursery - Pot Cuttings Stick Cuttings When Sat Oct 23 10am – 12pm Eastern Time Where 770 Wapping Road, Portsmouth, RI (map) Calendar ARS Massachusetts Chapter Calendar Who • John Perkins - creator

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Rhododendron decorum ssp. diaprepes 'Gargantua' is a Tetraploid

Rhododendron decorum ssp. diaprepes 'Gargantua', which is often documented as a triploid, has been determined to be a tetraploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron decorum ssp. diaprepes 'Gargantua' is known to be self fertile.

All elepidote Rhododendron species are documented to be diploids. Whether 'Gargantua' represents the sole anomaly from this rule is not known at this.

Summary of Ploidy Levels

Tetraploid Rhododendron decorum ssp. diaprepes 'Gargantua'

Source: Whitney Nursery
Source: Jim Barlup

John and Sally Perkins

Rhododendron maximum var. 'Leachii' is a Diploid

Rhododendron maximum var. 'Leachii' has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid Rhododendron maximum var. 'Leachii'

Source: Werner Brack

John and Sally Perkins

'Anita Gehnrich' is a Triploid

Rhododendron 'Anita Gehnrich', which is a Bud Gehnrich hybrid documented as 'The Honorable Jean Marie de Montague' X R. degronianum ssp. yakushimanum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Rhododendron 'Solidarity', which is documented as having the reversed parents of 'Anita Gehnrich', is also a triploid.

Summary of Ploidy levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Anita Gehnrich',
Triploid 'Solidarity'

Source: Werner Brack

John and Sally Perkins

Reminder: Massachusetts Chapter Meeting @ Wed Oct 20 7:30pm - 9:30pm (ARS Massachusetts Chapter Calendar)

more details » Massachusetts Chapter Meeting Meeting When Wed Oct 20 7:30pm – 9:30pm Eastern Time Where Elm Bank Wellsley, MA (map) Calendar ARS Massachusetts Chapter Calendar Who • John Perkins - creator

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'Hotei' is a Diploid

Rhododendron 'Hotei', which is a Karl Sifferman hybrid documented as 'Goldsworth Orange' x (souliei x wardii var. wardii), has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Hotei' is pollen sterile.

Summary of Ploidy Levels

Diploid 'Hotei'

Source Marc Colombel

John and Sally Perkins

Van Veen Order List and Instructions

Please download and print the attached Microsoft Word files to participate in the order for plants to be delivered in April 2011.

George Hibben

'Solidarity' is a Fertile Triploid

Rhododendron 'Solidarity', which is a Hank Schannen hybrid documented as R. degronianum ssp. yakushimanum X 'The Honorable Jean Marie de Montague', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Solidarity' is known, on occasion, to produce open pollinated seedpods and this seed will sometimes germinate.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Rhododendron 'Anita Gehnrich', which is documented as the reverse cross of 'Solidarity, is also a triploid.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Anita Gehnrich',
Triploid 'Solidarity'

Source: Werner Brack

John and Sally Perkins

Reminder: Board Meeting @ Wed Oct 13 7:30pm - 9pm (ARS Massachusetts Chapter Calendar)

more details » Board Meeting When Wed Oct 13 7:30pm – 9pm Eastern Time Where Hardings (map) Calendar ARS Massachusetts Chapter Calendar Who • John Perkins - creator

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'Horizon Lakeside' is a Diploid

Rhododendron 'Horizon Lakeside', which is a Ned Brockenbrough hybrid documented as ('Nancy Evans' x 'Lem's Cameo') X 'Skipper', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Horizon Lakeside',
Diploid 'Lem's Cameo',
Diploid 'Nancy Evans'

Source Marc Colombel

John and Sally Perkins

'Grace Seabrook' is a Triploid

Rhododendron 'Grace Seabrook', which is a Cecil Seabrook hybrid documented as 'The Honorable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Taurus', which is also documented as 'The Honorable Jean Marie de Montague' x R. strigillosum, has been determined to be a triploid using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch. 'Taurus', although reluctant to be a parent, appears to be both seed and pollen fertile.

Rhododendron 'The Honorable Jean Marie de Montague', although a diploid, is documented as the parent of several triploids indicating 'The Honorable Jean Marie de Montague' may throw more unreduced gametes than most diploids.

Summary of Ploidy Levels

Diploid 'The Honorable Jean Marie de Montague',
Triploid 'Grace Seabrook',
Triploid 'Taurus'

Source: Werner Brack

John and Sally Perkins

ARS Mass Chapter October Newsletter

Attached is the October Newsletter of the Am. Rhodo Society, Mass Chapter, courtesy of our Editor Susan Clark.  There are important announcements in this edition, so please be sure to take a look.

 

Notes on Van Veen program:

 

1. This year George Hibben will be organizing this popular program.  Communications regarding orders, etc. should be sent to him (his contact info is in this Newsletter).

 

2. A sentence included in the Van Veen announcement within this Newsletter should have been deleted.  To correct:  You do not need to send George your email address.  We have one master list to which an electronic version of the Van Veen order form will be sent.  Please do send to me any changes to your email address, however.

 

Joe Bruso

'Broughtonii' is a Fertile Triploid

Rhododendron 'Broughtonii', which is a 1840 Broughton hybrid documented as an arboreum hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Broughtonii' is the pollen parent to the famous 'Pink Pearl'.

Rhododendron 'Broughtonii' is the oldest known polyploid large elepidote hybrid.

Summary of Ploidy Levels

Triploid 'Broughtonii',
Triploid 'Pink Pearl'

Source Marc Colombel

John and Sally Perkins

'Loderi Venus' is a Diploid

Rhododendron 'Loderi Venus', which is a Sir Edmund Loder hybrid documented as R. fortunei ssp. fortunei x R. griffithianum, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Although Rhododendron griffithianum is in the background of several polyploid elepidote hybrids, many diploid hybrids also contain this species.

Summary of Ploidy Levels

Diploid 'Loderi Venus',
Triploid 'Markeeta’s Flame',
Triploid 'Markeeta’s Prize'

Source: Whitney Nursery

John and Sally Perkins

'Olin O. Dobbs' is a Diploid

Rhododendron 'Olin O. Dobbs', which is a Dobbs hybrid documented as 'Mars' x 'Purple Splendour', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Olin O. Dobbs'

Source: Greer Gardens

John and Sally Perkins

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'Mrs. Furnivall' is a Diploid

Rhododendron 'Mrs. Furnivall', which is a H. Waterer hybrid documented as Xgriffithianum x Xcaucasicum, has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Furnivall's Daughter',
Diploid 'Mrs. Furnivall'

Source: Whitney Nursery

John and Sally Perkins

'Hurricane' is a Diploid

Rhododendron 'Hurricane', which is a William Whitney hybrid documented as 'Mrs Furnivall' x 'Anna Rose Whitney', has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron 'Hurricane' has as ancestors 'Broughtonii', 'Cynthia', 'Pink Pearl', 'Countess of Derby', 'Mrs Furnivall', and 'Anna Rose Whitney'.

In the case of 'Hurricane', a diploid 'Mrs. Furnivall' crossed with a triploid 'Anna Rose Whitney' produced a diploid indicating 'Anna Rose Whitney' most likely contributed only a 1x gamete (13 chromosomes) to 'Hurricane'.

Summary of Ploidy Levels

Diploid 'Hurricane',
Diploid 'Mrs. Furnivall',
Triploid 'Anna Rose Whitney',
Triploid 'Broughtonii',
Triploid 'Cynthia',
Triploid 'Pink Pearl',
Tetraploid 'Countess of Derby'

Source: Whitney Nursery

John and Sally Perkins

'Peach Charm' is a Diploid

Rhododendron 'Peach Charm', which is a Ned Brockenbrough hybrid documented as 'Nancy Evans' X ('Lem's Whopper' X 'Lem's Cameo'), has been determined to be a diploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Summary of Ploidy Levels

Diploid 'Lem's Cameo',
Diploid 'Nancy Evans',
Diploid 'Peach Charm'

Source Marc Colombel

John and Sally Perkins

'Rwain' is a Fertile Triploid

Rhododendron 'Rwain', which is a Marc Colombel hybrid documented as 'Kerneostic' X 'Lem's Monarch', has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

The University of Coimbra research team also determined the Colombel cross of 'Horizon Monarch' by 'Rwain' produced a triploid and 3 tetraploids, 'Hotei' x 'Rwain' produced a triploid, the Colombel cross of 'Rwain' X 'L'Engin' produced 4 tetraploids, and the Colombel cross of 'Naselle' X 'Rwain' produced a diploid.

Thus, 'Rwain' is a seed and pollen fertile triplod capable of producing offspring that are diploids, triploids, and tetraploids. Although 'Rwain' is fertile, crosses involving 'Rwain' normally produce very few viable seeds.

These findings suggest that for the genus Rhododendrons triploids may provide a path for the exchange of generic material between diploids and tetraploids. To date the evidence suggesting a flow from diploid to tetraploid is much stronger than the evidence for a path from tetraploid to diploid.

Uneven bell curve splitting of fertile triploids allowing triploids to act as a donor of 1x or 2x gametes is demonstrated to occur in the research done in the thirties by Dr. Cyril Dean Darlington and in the nineties by Dr. Justin Ramsey.

These findings are consistent with the observations that Jim Barlup and Marc Colombel made when working with suspected polyploid elepidotes and the rules of engagement of John and Sally Perkins when working with deciduous azaleas.

'Lem's Monarch' and 'Horizon Monarch' have been determined to be tetraploids using flow cytometry by Dr. Thomas Ranney, J.R. Jones, and Nathan Lynch.

"Chromosomes and Plant Breeding", Macmillan (1932).

"Neopolyploidy in Flowering Plants" (2002).

"Let’s talk hybridizing: Hybridizing with elepidote polyploid rhododendrons." (2002)

"Rules of Engagement" (2009)

"Ploidy Levels and Relative Genome Sizes of Diverse Species, Hybrids, and Cultivars of Rhododendron" (2006)

Summary of Ploidy levels

Diploid 'Anna',
Diplid 'Countess of Athlone',
Diploid 'Hotei',
Diploid 'Naselle',
Diploid 'Norman Gill' ,
Diploid 'The Honourable Jean Marie de Montague',
Diploid 1 seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 seedling of 'Horizon Monarch' x 'Rwain',
Triploid 1 seedling of 'Hotei' x 'Rwain',
Tetraploid 'Horizon Monarch',
Tetraploid 'L'Engin',
Tetraploid 'Lem's Monarch',
Tetraploid 'Marnius Koster',
Tetraploid 3 seedlings of 'Horizon Monarch' x 'Rwain',
Tetraploid 4 seedlings of 'Rwain' X 'L'Engin'

Source Marc Colombel

Note: R W A I N is short for Rhododendron Without An Important Name

John and Sally Perkins