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Showing posts with label S_Appalachian. Show all posts
Showing posts with label S_Appalachian. Show all posts

H: Thursday, December 01, 2011

"viscosum glaucum not" X 'Smoky Mountaineer' is a Triploid

"viscosum glaucum not" X Rhododendron calendulaceum 'Smoky Mountaineer', which is a John and Sally Perkins hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In this case of this triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Triploid "viscosum glaucum not" X Rhododendron calendulaceum 'Smoky Mountaineer'
Tetraploid Rhododendron calendulaceum
Tetraploid Rhododendron calendulaceum 'Smoky Mountaineer'

This seedling was grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Wednesday, November 30, 2011

(pruniflorum X arborescens) X 'Smoky Mountaineer' is a Triploid

(Rhododendron pruniflorum X Rhododendron arborescens) X Rhododendron calendulaceum 'Smoky Mountaineer', which is a John and Sally Perkins hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron pruniflorum and Rhododendron arborescens are diploid species. Rhododendron calendulaceum is a tetraploid species.

In this case of this triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron pruniflorum,
Diploid Rhododendron arborescens,
Triploid (Rhododendron pruniflorum X Rhododendron arborescens) X Rhododendron calendulaceum 'Smoky Mountaineer',
Tetraploid Rhododendron calendulaceum,
Tetraploid Rhododendron calendulaceum 'Smoky Mountaineer'

This seedling was grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Monday, November 28, 2011

prinophyllum X calendulaceum Engine Gap is a Triploid

Rhododendron prinophyllum X Rhododendron calendulaceum "Engine Gap", which is a John and Sally Perkins hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron prinophyllum is diploid. Rhododendron calendulaceum is a tetraploid species.

In this case of this triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron prinophyllum,
Triploid Rhododendron prinophyllum X Rhododendron calendulaceum "Engine Gap",
Tetraploid Rhododendron calendulaceum

This seedling was grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Sunday, November 27, 2011

'Canobie Candy' X calendulaceum Engine Gap is a Triploid

'Canobie Candy' X Rhododendron calendulaceum "Engine Gap", which is a John and Sally Perkins hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

'Canobie Candy' is diploid. Rhododendron calendulaceum is a tetraploid species.

In this case of this triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid 'Canobie Candy',
Triploid 'Canobie Candy' X Rhododendron calendulaceum "Engine Gap",
Tetraploid Rhododendron calendulaceum

This seedling was grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Saturday, November 26, 2011

molle x luteum is a Triploid

Rhododendron molle X Rhododendron luteum, which is a John and Sally Perkins hybrid, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron molle is diploid species. Rhododendron luteum is a tetraploid species.

In this case of this triploid (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron molle,
Triploid Rhododendron molle X Rhododendron luteum,
Tetraploid Rhododendron luteum

This seedling was grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Tuesday, March 22, 2011

(cumberlandense X viscosum) X 'Apricot Surprise' is a Triploid

Two seedlings of [(Rhododendron cumberlandense X Rhododendron viscosum) X 'Apricot Surprise'], which is a John and Sally Perkins hybrid, have been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron cumberlandense and Rhododendron viscosum are diploid species. 'Apricot Surprise' is a tetraploid.

In the case of these 2 triploids (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron cumberlandense,
Diploid Rhododendron viscosum,
Triploid 2 seedlings from [(Rhododendron cumberlandense X Rhododendron viscosum) X 'Apricot Surprise' ],
Tetraploid 'Apricot Surprise'

Seedlings from this same cross were sold at the November 14, 2010 Founders Day Meeting of the ARS Massachusetts Chapter and at the November 20, 2010 Banquet of the New England Rock Garden Society.

These seedlings were grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins

H: Monday, November 15, 2010

[(cumberlandense X viscosum) X colemanii] is a Triploid

Two seedlings of [(Rhododendron cumberlandense X R. viscosum) X R. colemanii], which is a John and Sally Perkins hybrid, have been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

Rhododendron cumberlandense and Rhododendron viscosum are diploid species. Rhododendron colemanii is a tetraploid species.

In this case of these 2 triploids (39 chromosomes), a diploid crossed with a tetraploid produced a triploid indicating normal meiosis occurred where the diploid contributed a 1x gamete (13 chromosomes) and the tetraploid contributed a 2x gamete (26 chromosomes).

Summary of Ploidy Levels

Diploid Rhododendron cumberlandense,
Diploid Rhododendron viscosum,
Triploid 2 seedlings from [(Rhododendron cumberlandense X R. viscosum) X R. colemanii],
Tetraploid Rhododendron colemanii

Seedlings from this same cross were sold at the November 14, 2010 Founders Day Meeting of the ARS Massachusetts Chapter and at the November 20, 2010 Banquet of the New England Rock Garden Society.

These seedlings were grown on by

Lindy Johnson
Appalachian Native Plants
676 Waddell Road
PO Box 736
Mountain City, TN 37683

Source: John and Sally Perkins
Source: Appalachian Native Plants

John and Sally Perkins
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