H: Friday, August 12, 2011

'Hotei' x 'Rwain' is a Triploid

One seedling of Rhododendron 'Hotei' x 'Rwain', which was raised by Marc Colombel, has been determined to be a triploid using flow cytometry by

Dr. João Loureiro, Dr. Silvia Castro, José Cerca, and Mariana Castro
Plant Ecology and Evolution Group,
Centre for Functional Ecology,
Department of Life Sciences,
Faculty of Science and Technology,
University of Coimbra, Portugal.

In the case of the triploid seedling of 'Hotei' (2X) x 'Rwain' (3X), a diploid by a triploid produced a triploid indicating that triploid seed parent 'Rwain' acted as a tetraploid. In other words, the triploid seedlings of 'Hotei' x 'Rwain' having 39 chromosomes most likely received 13 chromosomes from the diploid 'Hotei' and 26 chromosomes from the triploid 'Rwain'.

Summary of Ploidy Levels

Diploid 'Hotei',
Diploid 'Naselle',
Diploid 1 Seedling of 'Naselle' X 'Rwain',
Triploid 'Rwain',
Triploid 1 Seedling of 'Hotei' x 'Rwain'

Source Marc Colombel

John and Sally Perkins
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